Mouse anti phosphotyrosine antibody clone 4g10

The following materials were purchased from the sources indicated: Dulbecco's Modified Eagle Medium (DMEM), CD293, fetal bovine serum (FBS), penicillin/streptomycin, Lipofectamine 2000, Geneticin and l-glutamine (Invitrogen); open reading frame (ORF) clone of human oviductal glycoprotein 1 (OVGP1, oviductin) (ORIGENE, Rockville, MD, USA); goat anti-oviductin antibody (P-20), oviductin immunizing peptide (P-20)P, horseradish peroxidase (HRP)linked donkey anti-goat IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP, fluorescein isothiocyanate (FITC)linked goat anti-mouse IgG and donkey anti-goat IgG-FITC (Santa Cruz Biotechnology); rabbit anti-human oviductal glycoprotein polyclonal antibody was a gift from Dr Patricia Mavrogianis, University of Illinois, Chicago, IL, USA (O' Day-Bowman et al. 1996) ; mouse anti-phosphotyrosine antibody (clone 4G10) (EMD Millipore); mouse anti-α-tubulin antibody (clone B-5-1-2) (Sigma-Aldrich); Western Lighting-enhanced Chemiluminescence Substrate, Micromass MassPREP Robotic Protein Handling System (PerkinElmer); Amicon Ultra-4 and -15 centrifugal filter units with a 30 kDa molecular weight cutoff (EMD Millipore); Superdex 200 (XK 16/60) and GammaBind plus Sepharose (GE Healthcare Bio-Sciences AB). All other chemicals were obtained from Sigma-Aldrich.