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Rabbit anti flotillin 1

Manufactured by Proteintech

Rabbit anti-Flotillin 1 is a primary antibody that recognizes the Flotillin 1 protein. Flotillin 1 is a membrane-associated protein involved in the formation of lipid rafts and cellular signaling.

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2 protocols using rabbit anti flotillin 1

1

Comprehensive Antibody Reagents for Influenza A and IBDV Detection

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The antibodies and reagents used in this study were commercially obtained from different sources, which were listed as follows: mouse anti-Influenza A NP, mouse anti-Influenza A NS1 and mouse anti-ISG15 (Santa Cruz); rabbit anti-F4/80 antibody and mouse anti-β-Tubulin (Abcam); rabbit anti-ISG15, rabbit anti-RIG-I, rabbit anti-MAVS, rabbit anti-MDA5, Alexa Fluor 488-cunjugated anti-Rabbit IgG (H+L), F(ab’)2 Fragment and anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor 647 Conjugate), anti-rabbit IgG (H+L) (DyLight 680 Conjugate), anti-rabbit IgG (H+L) (DyLight 800 4X PEG Conjugate) and anti-mouse IgG (H+L) (DyLight 680 Conjugate) (CST); rabbit anti-RIG-I, mouse anti-Flag tag, mouse anti-AIF, rabbit anti-CD71, rabbit anti-Flotillin 1 and mouse anti-GAPDH (Proteintech); rabbit anti-ADAP (Millipore); mouse anti-ADAP (BD); mouse anti-HA tag (Sigma-Aldrich); IFN-β Protein (Sino Biology); LPS derived from Escherichia coli strain O111:B4 (Sigma-Aldrich); poly (I:C) (Selleck Chemicals). Mouse anti-viral protein (VP3) of IBDV polyclonal antibody was generated from the sera of mice by immunization with purified IBDV.
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2

Plasma-derived Extracellular Vesicle Characterization

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Plasma-derived EVs were isolated by sequential ultracentrifugation.22 (link),23 Typical EV markers used were rabbit-anti-CD63 (Santa Cruz Biotechnology, SC-15363), mouse-anti-CD9 (Proteintech, 60232-1-Ig), and rabbit-anti-Flotillin-1 (Proteintech, 15571-1-AP). Albumin was visualized by Ponceau S staining. The size distribution and concentration of EVs were measured by nanoparticle tracking analysis (NTA) (Particle Metrix). Transmission electron microscopy (TEM) (FEI Company) with negative staining was performed to characterize EV morphology.
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