Mitoxantrone hydrochloride

Mitoxantrone hydrochloride was purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China) Recombinant mouse GM-CSF and IL-4 were obtained from Peprotech (Rocky Hill, NJ, USA). ATP assay system bioluminescence detection kit was purchased from Promega Corporation (Madison, WI, USA). Mouse HMGB1 ELISA kit was purchased from Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China). Anti-calreticulin antibody and goat anti-rabbit IgG H&L (FITC) were obtained from Abcam (Cambridge, UK). 4-Dodecyl aniline (99.9%), phenol (99.9%), 1, 3-dibromopropane (99.9%), diethylamine (99.9%), hydrochloric acid (HCI, 37%), etc. were purchased from Chron Chemicals Co., Ltd. (Chengdu, China). Sodium nitrite (NaNO₂, >98%), potassium iodide (KI, > 98%), potassium carbonate (K₂CO₃, >98%), dithionite (Na₂S₂O₄, >98%), and β-mercaptoethanol, etc. were purchased from Macklin Co., Ltd. (Shanghai, China). Cholesterol (CHOL), DSPE-mPEG2000, and DSPE-PEG2000-NHS were obtained from Advanced Vehicle Technology Co., Ltd. (Shanghai, China). DiD was obtained from Bosunlife Biotechnology Co., Ltd. (Beijing, China). Antibodies used in flow cytometry analysis were purchased from Biolegend (San Diego, CA, USA), Abcam (Cambridge, UK), Bioss Co., Ltd. (Beijing, China), or eBioscience (San Diego, CA, USA). Detailed sources of all antibodies are provided in Supporting Information Table S1.