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2 protocols using serotonin d4 β d glucuronide

1

Glucuronidation of Pharmaceuticals and Metabolites

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Chemicals EC1.5-P-and FH-P-SWCNTs were obtained from Meijo Nano Carbon Co., Ltd. (Nagoya, Japan). Alamethicin, β-estradiol, β-estradiol 3-(β-D-glucuronide) sodium salt, carbon black, fullerene-C 60 , and fullerene-C 70 , imipramine hydrochloride, serotonin hydrochloride, and uridine 5ʹ-diphosphoglucuronic acid (UDPGA) trisodium salt were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Imipramine N-β-D-glucuronide, propofol, serotonin β-Dglucuronide, and serotonin-d4 β-D-glucuronide were obtained from Toronto Research Chemicals (Toronto, Canada). Furthermore, propofol β-D-glucuronide was acquired from the Cayman Chemical Company (Ann Arbor, MI, U.S.A.).
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2

Acetaminophen Glucuronidation Assay Development

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Materials Acetaminophen (APAP) was purchased from Nacalai Tesque (Kyoto, Japan). Alamethicin, APAP β-Dglucuronide, β-estradiol, β-estradiol 3-(β-D-glucuronide) sodium salt, serotonin hydrochloride, and uridine 5′-diphosphoglucuronic acid trisodium salt were obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.). Serotonin β-D-glucuronide and serotonin-d 4 β-D-glucuronide were purchased from Toronto Research Chemicals (Toronto, Canada). Carbamazepine (CBZ), β-naphthoflavone (BNF), and phenobarbital (PB) were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other chemicals and solvents were of the highest commercial grade available.
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