After being treated with appropriate doses of each drug for indicated time, cell samples, including GH3, MMQ, and primary pituitary tumor cells, were harvested in sterile Eppendorf tubes and washed with cold PBS for two times. Then, these samples were lysed in lysis buffer (50 mM Tris [pH 7.5], 120 mM NaCl, and 0.5% NP-40) containing protease and phosphatase inhibitors and placed on ice for 30 minutes. After that, they were centrifuged at 12,000 × g for 10 minutes. The total protein concentration of the samples was measured using the bicinchoninic acid protein assay kit (Tiangen Biotech, PA115). The protein samples were separated by SDS-PAGE and immunoblotted with the indicated antibodies. The LAS4000 system was used for imaging, and protein band intensity was quantified by densitometry using the ImageJ software. The following antibodies were used in this study: Tubulin antibody (11224-1-AP; Proteintech Group), S6K1 (Cat#2708, Cell Signaling Technology), p-S6K1-Thr389 (Cat#9234, Cell Signaling Technology), 4EBP1 (Cat#9644, Cell Signaling Technology), p-4EBP1 (Cat#2855, Cell Signaling Technology), Antirabbit IgG, HRP-linked antibody and Anti-mouse IgG, and HRP-linked antibody (#7076; #7074, Cell Signaling Technology).
Tubulin antibody 11224 1 ap
Manufactured by Proteintech
Sourced in United States
The Tubulin antibody (11224-1-AP) is a primary antibody that specifically binds to the tubulin protein. Tubulin is a structural protein that is a major component of the cytoskeleton and is involved in various cellular processes such as cell division and intracellular transport.
Automatically generated - may contain errors
Lab products found in correlation
Bicinchoninic acid protein assay kit, by Tiangen Biotech (2 mentions)
Hrp linked antibody, by Cell Signaling Technology (1 mentions)
P s6k1 thr389, by Cell Signaling Technology (1 mentions)
4e bp1, by Cell Signaling Technology (1 mentions)
P 4ebp1, by Cell Signaling Technology (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Imagequant las 4000, by Thermo Fisher Scientific (1 mentions)
Atg7 antibody, by Cell Signaling Technology (1 mentions)
Lc3 antibody, by Cell Signaling Technology (1 mentions)
Pierce ecl chemiluminescence reagents, by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
2 protocols using tubulin antibody 11224 1 ap
1
Immunoblot Analysis of Pituitary Cell Proteins
2
Western Blot Analysis of Autophagy Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were sued in this study: ATG7 antibody (#8558; Cell Signaling Technology), LC3 antibody (#3868; Cell Signaling Technology), BECN1 antibody (#3738; Cell Signaling Technology), tubulin antibody (11224-1-AP; Proteintech Group, Chicago, IL, USA); caspase-8 antibody (13423-1-AP; Proteintech Group); antirabbit IgG, horseradish peroxidase (HRP)-linked antibody (#7074; Cell Signaling Technology) and anti-mouse IgG, HRP-linked antibody (#7076; Cell Signaling Technology). Cells were lysed with the radioimmunoprecipitation assay (RIPA) buffer (P0013C; Beyotime Biotechnology), and the total protein concentration was measured using the Bicinchoninic Acid Protein Assay Kit (PA115; Tiangen Biotech). In addition, proteins from whole-cell extracts were resolved using denaturing SDS-PAGE and analyzed by Western blotting. Subsequently, equal amounts of protein were separated using SDS-PAGE and transferred to polyvinylidene fluoride membranes (Millipore). Protein bands were detected with Pierce ECL chemiluminescence reagents (Thermo Fisher) and exposed on the ImageQuant LAS 4000 (Japan) system. Immunoblots shown in figures were derived from three independent experiments. Furthermore, intensities of protein bands were quantified by densitometry using the ImageJ software (http://imagej. nih.gov/ij/).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!