HEK 293F (HEK) cells (Thermo Fisher Scientific) were cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific) supplemented with penicillin-streptomycin (0.2 U/mL) (Thermo Fisher Scientific). CHO-S cells (Thermo Fisher Scientific) were cultured in FreeStyle CHO Expression Medium (Thermo Fisher Scientific) supplemented with L-glutamine (8 mM, Thermo Fisher Scientific) and penicillin-streptomycin (0.2 U/mL). All cells were grown at 37°C and 5% CO2 with rotation at 125 rpm in a humidified atmosphere. Transfections were incubated in the same growth conditions. HEK cells were not subject to more than 25 passages, whereas CHO cells were studied below 10 passages to mitigate cell clumping. All cells were grown to a density of 1.2 × 106 cells/mL and diluted to 1.0 × 106 cells/mL on the day of transfection.
Hek 293f hek cells
Manufactured by Thermo Fisher Scientific
HEK 293F (HEK) cells are a widely used cell line derived from human embryonic kidney cells. They are commonly used in various research applications, including the production and study of recombinant proteins, gene expression analysis, and viral vector production.
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Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Freestyle 293 expression medium, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Cho s cells, by Thermo Fisher Scientific (2 mentions)
Freestyle cho expression medium, by Thermo Fisher Scientific (2 mentions)
2 protocols using hek 293f hek cells
1
Culturing and Transfecting HEK 293F and CHO-S Cells
2
Culturing HEK 293F and CHO-S cells
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HEK 293F (HEK) cells (Thermo Fisher Scientific) were cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific) supplemented with penicillin-streptomycin (0.2 U/mL) (Thermo Fisher Scientific). CHO-S cells (Thermo Fisher Scientific) were cultured in FreeStyle CHO Expression Medium (Thermo Fisher Scientific) supplemented with L-glutamine (8 mM, Thermo Fisher Scientific) and penicillin-streptomycin (0.2 U/mL). All cells were grown at 37°C and 5% CO 2 with rotation at 125 rpm in a humidified atmosphere. Transfections were incubated in the same growth conditions. HEK cells were not subject to more than 25 passages, whereas CHO cells were studied below 10 passages to mitigate cell clumping. All cells were grown to a density of 1.2×10 6 cells/mL and diluted to 1.0×10 6 cells/mL on the day of transfection.
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