The U-2 OS-GFP cells were seeded in a 24-well plate at a cellular density of 25,000 cells/well and treated with different concentrations of GFP38CRISPR-RhB-MSNs (0, 25, 50, and 100 µg/mL). Cells were incubated for 24 h and 48 h, and viability was determined by adding cell proliferation reagent WST-1 (Sigma Aldrich) according to manufacturer’s instructions. After 1 h of incubation, absorbance at 595 nm was measured in a Wallac 1420 workstation.
THP-1 cells were seeded in a p96-well plate at a cellular density of 10,000 cells/well and treated with different concentrations of GSDMD45CRISPR-RhB-MSNs (0, 25, 50, and 100 µg/mL). Cells were incubated for 24 and 48 h, and then cell viability was determined by the WST-1 assay and inflammatory cell death by LDH activity assay (Promega, G7891, Madrid, Spain). For LDH activity, cell supernatants were analyzed by following the manufacturer’s instructions. As a positive control, 1% Triton-X100 was added.
THP-1 cells were seeded in a p96-well plate at a cellular density of 10,000 cells/well and treated with different concentrations of GSDMD45CRISPR-RhB-MSNs (0, 25, 50, and 100 µg/mL). Cells were incubated for 24 and 48 h, and then cell viability was determined by the WST-1 assay and inflammatory cell death by LDH activity assay (Promega, G7891, Madrid, Spain). For LDH activity, cell supernatants were analyzed by following the manufacturer’s instructions. As a positive control, 1% Triton-X100 was added.