Dicoumarol m1390

Dulbecco’s modified Eagle’s medium (DMEM with 25 mM glucose) powder and penicillin/streptomycin solution was obtained from Gibco/Invitrogen (Darmstadt, Germany) and fetal calf serum (FCS) from Sigma-Aldrich (Steinheim, Germany). β-lapachone (ab141097) was purchased from Abcam (Berlin, Germany), dicoumarol (M1390) and iodoacetate (I6375) were from Sigma-Aldrich (Steinheim, Germany). ES936 (sc-362737) was obtained from Santa Cruz Biotechnology (Heidelberg, Germany), WST1 (W201) from Dojindo (Munich, Germany) and G6PDi-1 (31484) from Cayman Chemical (Tallinn, Estonia). The enzymes for the metabolite assays were purchased from Roche Diagnostics (Mannheim, Germany). All other chemicals of the highest purity available were purchased from Merck (Darmstadt, Germany), Sigma-Aldrich (Steinheim, Germany) or AppliChem (Darmstadt, Germany). Sterile cell culture material and non-sterile microtiter plates were from Sarstedt (Nümbrecht, Germany).

β-lap (ab141097) was purchased from Abcam (Berlin, Germany) and dicoumarol (M1390) from Sigma-Aldrich (Steinheim, Germany). Dulbecco’s modified Eagle’s medium (DMEM with 25 mM glucose) and penicillin/streptomycin solution was obtained from Gibco (Darmstadt, Germany) and fetal calf serum from Biochrom (Berlin, Germany). Bovine serum albumin (BSA), NADPH, NADH and sulfosalicylic acid were from AppliChem (Darmstadt, Germany) and the enzyme glutathione reductase was purchased from Roche Diagnostics (Mannheim, Germany) and from Sigma-Aldrich (Steinheim, Germany). All other chemicals of the highest purity available were purchased from Merck (Darmstadt, Germany), Sigma-Aldrich (Steinheim, Germany), Fluka (Buchs, Switzerland), Dojindo (Munich, Germany), Roche (Mannheim, Germany), Roth (Karlsruhe, Germany) or Riedel-de Haën (Seelze, Germany). Sterile cell culture plates and non-sterile normal and black 96-well microtiter plates were from Sarstedt (Nümbrecht, Germany).