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Raw blue isg

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RAW-Blue ISG is a cell line engineered to report on the activation of the interferon-stimulated gene (ISG) pathway. The cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an ISG promoter, allowing for the quantitative assessment of ISG induction.

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Lab products found in correlation

Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Nonessential amino acid, by Corning (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions) B16 blue isg, by InvivoGen (2 mentions) Thp1 blue isg, by InvivoGen (2 mentions)

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RAW-Blue (17 citations) RAW-Blue ISG cells (2 citations)

2 protocols using raw blue isg

1

Culturing Immune Cell Lines for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
DC2.4 cells were kindly provided by Prof. Kenneth Rock (University of Massachusetts Medical School) and cultured in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/mL penicillin (Gibco), 100 μg/mL streptomycin (Gibco), 2 mM L-glutamine, 50 μM 2-mercaptoethanol (Gibco), 1x nonessential amino acids (Cellgro), and 10 mM HEPES (Invitrogen). B16-Blue ISG, THP-1-Blue ISG, and RAW-Blue ISG cells were purchased from Invivogen, and cultured according to manufacturer specifications. No authentication of the cell lines were performed by the authors. All cells lines were tested for mycoplasma contamination and grown in a humidified atmosphere with 5% CO2 at 37°C.
Shae D., Becker K.W., Christov P., Yun D.S., Lytton-Jean A.K., Sevimli S., Ascano M., Kelley M., Johnson D.B., Balko J.M, & Wilson J.T. (2019). Endosomolytic Polymersomes Increase the Activity of Cyclic Dinucleotide STING Agonists to Enhance Cancer Immunotherapy. Nature nanotechnology, 14(3), 269-278.
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2

Culturing Immune Cell Lines for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
DC2.4 cells were kindly provided by Prof. Kenneth Rock (University of Massachusetts Medical School) and cultured in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/mL penicillin (Gibco), 100 μg/mL streptomycin (Gibco), 2 mM L-glutamine, 50 μM 2-mercaptoethanol (Gibco), 1x nonessential amino acids (Cellgro), and 10 mM HEPES (Invitrogen). B16-Blue ISG, THP-1-Blue ISG, and RAW-Blue ISG cells were purchased from Invivogen, and cultured according to manufacturer specifications. No authentication of the cell lines were performed by the authors. All cells lines were tested for mycoplasma contamination and grown in a humidified atmosphere with 5% CO2 at 37°C.
Shae D., Becker K.W., Christov P., Yun D.S., Lytton-Jean A.K., Sevimli S., Ascano M., Kelley M., Johnson D.B., Balko J.M, & Wilson J.T. (2019). Endosomolytic Polymersomes Increase the Activity of Cyclic Dinucleotide STING Agonists to Enhance Cancer Immunotherapy. Nature nanotechnology, 14(3), 269-278.
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