Antibodies against p smad2

Murine recombinant IL-4 was obtained from PeproTech (London, UK). Antibodies against collagen type I was purchased from EMD Millipore (Schwalbach, Germany). Antibodies against fibronectin, α-SMA, arginase-1, CD68, MBD2, and pro-SPC were purchased from Abcam (Cambridge, MA, USA), and antibodies against p-Smad2, p-Smad3, STAT6, P85, p-P85, and p-Akt (Thr³⁰⁸) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against MBD2, glyceraldehyde-3-phosphate dehydrogenase, p-Akt (Ser⁴⁷³), and p-STAT6 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse F4/80-PerCP/Cy5.5 and CD206–fluorescein isothiocyanate (FITC) were from BioLegend (San Diego, CA, USA), and CD11c–allophycocyanin (APC) were purchased from BD Biosciences (San Jose, CA, USA). Lipidoid (C12-200) was purchased from Xinjiahecheng Medical Chemistry Corporation (Wuhan, Hubei, China). mPEG2000-DEG was purchased from NOF Corporation (Tokyo, Japan). All other reagents were purchased from Sigma-Aldrich (St Louis, MO, USA), unless otherwise stated.

Minimal Essential Media (MEM), Fibroblast Expansion Media and Fibroblast Growth factor, Fetal Bovine Serum (FBS) and l-glutamine, phosphate buffer saline (PBS) were purchased from HiMedia (Mumbai, India). Penicillin streptomycin, MEM NEAA, MEM amino acids, MEM vitamin solution were obtained from Life Technologies, USA. Antibody against collagen-1,collagen-3, CTGF, α-SMA, anti-mouse HRP linked secondary antibody, anti-rabbit HRP linked secondary antibody were purchased from Abcam (MA, USA). Antibodies against TGF-β, IFN-γ, VEGF, p-AkT, Actin were purchased from Santa Cruz Biotechnology (Dallas, Texas USA). Antibodies against p-SMAD2, p-SMAD3, p-FAK, FAK, AkT were purchased from Cell Signaling Technology (Danvers, MA, USA). Vectashield mounting medium was from Vector Laboratories, Inc. (CA, USA). MTT 3-(4, 5-dimethylthiazol-2-yl) −2, 5-diphenyltetrazolium bromide), Bovine serum albumin (BSA) were purchased from SRL India. Streptozotocin, Ketamine and Xylazine were purchased from Sigma-Aldrich, India.