IHC was performed on para n-embedded sections. The sections were depara nized in xylene and hydrated with decreasing concentrations of ethanol (100, 90, 80, 75%) for 3 min each time and microwaved-heated in sodium citrate buffer for antigen retrieval. Then, the sections were blocked in 5% BSA and incubated with anti-IGF2BP3, VEGF, CD31, Ki67 rabbit polyclonal antibody (1:200; ProteinTech Group, Inc., Wuhan, China) at 4°C overnight. Next, the sections were treated with horseradish peroxidase (HRP)-conjugated rabbit secondary antibody (1:200; ProteinTech Group, Inc.) for 60 min at room temperature; then, 3,3'-diaminobenzidine development (DAB Substrate Chromogen System; Dako, Denmark) and hematoxylin staining were performed. The sections were xed and images were obtained with inverted microscope (Olympus IX71, Japan).
67 rabbit polyclonal antibody
Manufactured by Proteintech
The 67 rabbit polyclonal antibody is a laboratory research tool designed to detect and identify specific target proteins in biological samples. This antibody is produced by immunizing rabbits with a designated antigen, resulting in a population of antibodies that recognize multiple epitopes on the target protein. The 67 rabbit polyclonal antibody can be used in various immunoassay techniques, such as Western blotting and immunohistochemistry, to facilitate the study of protein expression and localization.
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2 protocols using 67 rabbit polyclonal antibody
1
Immunohistochemical Evaluation of Cancer Markers
2
Immunohistochemistry Profiling of Tumor Markers
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IHC was performed on para n-embedded sections. The sections were depara nized in xylene and hydrated with decreasing concentrations of ethanol (100, 90, 80, 75%) for 3 min each time and microwaved-heated in sodium citrate buffer for antigen retrieval. Then, the sections were blocked in 5% BSA and incubated with anti-IGF2BP3, VEGF, CD31, Ki67 rabbit polyclonal antibody (1:200; ProteinTech Group, Inc., Wuhan, China) at 4 °C overnight. Next, the sections were treated with horseradish peroxidase (HRP)-conjugated rabbit secondary antibody (1:200; ProteinTech Group, Inc.) for 60 min at room temperature; then, 3,3'-diaminobenzidine development (DAB Substrate Chromogen System; Dako, Denmark) and hematoxylin staining were performed. The sections were xed and images were obtained with inverted microscope (Olympus IX71, Japan).
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