After inoculating the strains from glycerol stocks in nutrient agar (2 mL), they were grown at 37 ºC overnight. Next, the strains were sub-cultured in the nutrient medium (5 mL) and grown to the mid-exponential phase (OD600= 1.5-2.0). Afterward, an aliquot of the culture (0.25 mL) was added to 2 mL of RNX-plus (Sinaclon, Iran). In addition, isolation of total RNA was performed based on the manufacturer's guidelines. DNase treatment was applied to remove the residual DNA with 20 U of RQ1 DNase I (Sinaclon, Iran).
Rq1 dnase 1
Manufactured by Sinaclon
RQ1 DNase I is a laboratory reagent used to degrade DNA. It functions by catalyzing the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, effectively breaking down DNA molecules.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rq1 dnase 1
1
Bacterial Culturing and RNA Extraction
2
RNA Extraction from Bacterial Cultures
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After inoculating the strains from glycerol stocks in nutrient agar (2 mL), they were grown at 37ºC overnight. Then, the strains were sub-cultured in the nutrient medium (5 mL) and grown to the mid-exponential phase (OD600=1.5-2.0). Afterward, an aliquot of the culture (0.25 mL) was added to 2 mL of RNX-plus (Sinaclon, Iran). In addition, isolation of total RNA was performed based on the manufacturer's guidelines. DNase treatment was applied to remove the residual DNA with 20 U of RQ1 DNase I. (Sinaclon, Iran).
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