0.45 μm syringe filters

Manufactured by Pall Corporation

Sourced in United States

The 0.45 μm syringe filters are a type of laboratory equipment used for filtration. They have a pore size of 0.45 micrometers and are designed to filter liquids through a membrane.

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Lab products found in correlation

Purinomycin, by Thermo Fisher Scientific (1 mentions) Halt protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

0.45 μm filter (16 citations) Syringe filters (10 citations)

2 protocols using 0.45 μm syringe filters

Cloning and Lentiviral Transduction of PD-L1 and KRAS

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Full‐length human PD‐L1‐coding cDNA with HA and FLAG labels was PCR amplified from CS‐U0767‐pIRES and CS‐U0767‐M83 (purchased from GeneCopoeia Company, Guangzhou, China). Full‐length and truncated domains of human KRAS coding cDNA were PCR amplified from LS 174 T, and the domain of 3x‐flag cDNA was PCR amplified and cloned into the pSIN‐EF‐Puro vector. Primers for PCR are in the Table S1.
All lentiviruses were packaged in 293 T cells with psPAX2 and pMD2. G, purified and concentrated using 0.45 μm syringe filters (Pall Corporation, Port Washington, NY, USA), aliquoted and stored at −80 °C. Cells were infected with concentrated lentivirus solution at a final concentration of 1/50 and screened with 3 μg/mL purinomycin (Thermo Fisher Scientific).

Cao Y., Liang W., Fang L., Liu M., Zuo J., Peng Y., Shan J., Sun R., Zhao J, & Wang J. (2022). PD‐L1/PD‐L1 signalling promotes colorectal cancer cell migration ability through RAS/MEK/ERK. Clinical and Experimental Pharmacology & Physiology, 49(12), 1281-1293.

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Extracellular Protein Isolation from Pseudomonas

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Pseudomonas aeruginosa PA14 was grown in MINS for 18 h at 37°C with shaking and good aeration, reaching an A600 of approximately 1.0 (late exponential phase–early stationary phase). Then, Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific, Waltham, MA) was added to bacterial cultures. The cells were removed by centrifugation at 5,000 × g for 15 min at 4°C, and supernatants were filtered using 0.45 μm syringe filters (Pall Corporation) to remove bacteria debris. Cell-free supernatants were concentrated using 3 K MWCO 20 mL devices (Pall Corporation) and by centrifugation at 5,000 g for 30 min at 17°C. Concentrated samples were washed and desalted in the same devises by adding 10 mL 50 mM ammonium bicarbonate and centrifugation at 5,000 g for 20 min at 17°C; this step was repeated three times. Last, the samples were concentrated at 5,000 g for 1 h at 17°C, resulting in a final volume of approximately 1 mL extracellular protein fraction.

Ballante F., Turkina M.V., Ntzouni M., Magnusson K.E, & Vikström E. (2023). Modified N-acyl-L-homoserine lactone compounds abrogate Las-dependent quorum-sensing response in human pathogen Pseudomonas aeruginosa. Frontiers in Molecular Biosciences, 10, 1264773.

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