Plant rna rapid extraction kit

Manufactured by Mei5 Biotechnology

Sourced in China

The Plant RNA Rapid Extraction Kit is a laboratory product designed to efficiently extract RNA from plant samples. The kit utilizes a rapid and streamlined protocol to isolate high-quality RNA suitable for downstream applications such as reverse transcription and real-time PCR analysis.

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RNA extraction kit (2 citations)

2 protocols using plant rna rapid extraction kit

Quantification of Plant Immune Genes

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Total RNA was isolated from samples according to the instructions provided with the Plant RNA Rapid Extraction Kit (Mei5 Biotechnology, Beijing, China). The quantity and quality of total RNA were tested by gel electrophoresis and spectrophotometry. Genes selected for further analysis included PR1.1, PR2, PR3, and PR4, with the internal control being the glyceraldehyde-3-phosphate dehydrogenase gene from T. aestivum (TaGAPDH). The primer sequences are listed in Table S1. All procedures for RT-qPCR were conducted as mentioned above.

Yao Y., Kan W., Su P., Zhu Y., Zhong W., Xi J., Wang D., Tang C, & Wu L. (2022). Hydrogen sulphide alleviates Fusarium Head Blight in wheat seedlings. PeerJ, 10, e13078.

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Arabidopsis ABA Responsiveness Assay

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The seeds of Col-0, OE PIA1 (OE-1, OE-2), and pia1 were grown vertically on 1/2 MS solid medium for 14 days. Then, 15 seedlings were transferred to 1/2 MS liquid medium for 12 h and treated with 3 μM ABA or water for 5 h. Experiments were performed under the instructions of a plant RNA rapid extraction kit (Mei5bio, Beijing, China). Reverse transcription was performed according to the instructions of hiscript III RT Supermix for the qPCR (+ gDNA wiper) kit (Vazyme). Primers are listed in Table S1.

Huang Y., Yang R., Luo H., Yuan Y., Diao Z., Li J., Gong S., Yu G., Yao H., Zhang H, & Cai Y. (2023). Arabidopsis Protein Phosphatase PIA1 Impairs Plant Drought Tolerance by Serving as a Common Negative Regulator in ABA Signaling Pathway. Plants, 12(14), 2716.

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