Cell counter

Manufactured by Revvity

Sourced in United States

The Revvity Cell Counter is a device used to count and analyze cells in a sample. It provides precise and accurate cell counts, enabling researchers and scientists to monitor cell populations and track changes over time. The core function of the cell counter is to accurately determine the number of cells present in a given volume of a sample.

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Lab products found in correlation

Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (2 mentions) Ultrapure agar, by Thermo Fisher Scientific (1 mentions) Falcon cell culture insert, by BD (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) L lactic acid colorimetric assay kit, by Elabscience (1 mentions) Zombie aqua dye, by BioLegend (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Dnase 1, by Merck Group (1 mentions) P yap, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Advanced dmem f12, by Thermo Fisher Scientific (1 mentions) Collagenase 8, by Merck Group (1 mentions) Trizol reagent, by Merck Group (1 mentions) Trypan blue, by Thermo Fisher Scientific (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) L glutamate, by Thermo Fisher Scientific (1 mentions) 2 mercaptoethanol bme, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Automacs, by Miltenyi Biotec (1 mentions) Axiocam mrm, by Zeiss (1 mentions)

Close spelling variants of this product

Automated cell counter Cellometer Automated Cell Counter Cellometer Auto T4 Plus Cell Counter Cellometer K2 cell counter Cellometer Auto 2000 Cell Viability Counter Cellometer Auto T4 Cell Viability Counter Cellometer Mini Automated Cell Counter Nexcelom Cellometer Auto 2000 Cell Viability Counter Nexcelom Cellometer Auto T4 cell counter Cellometer Auto X4 Cell Counter

19 protocols using cell counter

Magnetic nanoparticle tumor targeting

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FVB/N female mice (three animals per group with two tumors each) growing huHER2 allograft tumors at ~100 mm³ received intravenous (tail vein) injections of BP or BH (5 mg of Fe per mouse). Mice were euthanized 24 hours after injection. Tumors were processed as above for flow cytometry and placed on permanent magnet for 30 min. After discarding supernatant, total numbers of magnetically attached cells were counted in a cell counter (Nexcelom, MA).

Korangath P., Barnett J.D., Sharma A., Henderson E.T., Stewart J., Yu S.H., Kandala S.K., Yang C.T., Caserto J.S., Hedayati M., Armstrong T.D., Jaffee E., Gruettner C., Zhou X.C., Fu W., Hu C., Sukumar S., Simons B.W, & Ivkov R. (2020). Nanoparticle interactions with immune cells dominate tumor retention and induce T cell–mediated tumor suppression in models of breast cancer. Science Advances, 6(13), eaay1601.

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Quantitative Cell Proliferation and Migration Assays

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For proliferation assays, 24 h after transfection, 0.5X10⁴ cells were plated in 6-well plates in triplicate, and cells were quantified in a cell counter (Nexcelom) at the indicated times. The transwell cell migration assays were performed in duplicate, using 6.5 mm diameter Falcon cell culture inserts (8 mM pore size, Falcon) in 24 well cell culture plates. 1X10⁵ cells in serum-free media were transferred to the upper chamber, while the lower chamber contained media with 10% FBS. Following overnight incubation, the cells remaining on the upper surface were removed with a cotton swab. Migrated cells were fixed, visualized microscopically, photographed, and quantified using Image J. For soft agar assays, 1X10⁵ stably transfected cells were mixed with complete media containing 0.4% of ultrapure agar (Invitrogen) and placed over 0.6% basal agar in 60 mm dishes. 3 to 5 plates per transfectant were used. Cells were grown for 3–4 weeks, and colonies were stained with Nitrotetrazolium Blue Chloride (1 mg/ml), photographed by microscopy, and quantified with a colony counter.

Sanchez-Solana B., Wang D., Qian X., Velayoudame P., Simanshu D.K., Acharya J.K, & Lowy D.R. (2021). The tumor suppressor activity of DLC1 requires the interaction of its START domain with Phosphatidylserine, PLCD1, and Caveolin-1. Molecular Cancer, 20, 141.

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Hepatocyte Incubation and Analysis

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CD-1 male mouse hepatocytes (BioreclamationIVT, M005052, lot:LRH) were thawed and warmed to 37 °C using Lebovitz’s L-15 medium and Cell yield/cell density and viability were assessed by a Nexcelom cell counter.
Incubations were conducted on a Hamilton robot, 0.25 mL of the hepatocyte suspension (1 million cells/mL) was transferred for each incubation into Nunc 96-deepwell plate. Substrate (2.5 µL of 0.5 mM in acetonitrile) was added (final concentration of 5 µM) and then incubated for 60 minutes at 37 °C. The samples were quenched with an equal volume of acetonitrile, spun at 3500 rpm for 10 minutes and 0.2 mL of the supernatant was transferred into 0.6 mL of water for analysis by UHPLC-UV-HRAMS.

Hayhow T.G., Williamson B., Lawson M., Cureton N., Braybrooke E.L., Campbell A., Carbajo R.J., Cheraghchi-Bashi A., Chiarparin E., Diène C.R., Fallan C., Fisher D.I., Goldberg F.W., Hopcroft L., Hopcroft P., Jackson A., Kettle J.G., Klinowska T., Künzel U., Lamont G., Lewis H.J., Maglennon G., Martin S., Gutierrez P.M., Morrow C.J., Nikolaou M., Nissink J.W., O’Shea P., Polanski R., Schade M., Scott J.S., Smith A., Weber J., Wilson J., Yang B, & Crafter C. (2024). Metabolism-driven in vitro/in vivo disconnect of an oral ERɑ VHL-PROTAC. Communications Biology, 7, 563.

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Knockdown of GPER in Cell Lines

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siRNAs against GPER were purchased from Santa Cruz Biotechnology (GPER-a) and Thermo Fisher Scientific (GPER-b) and transfected into the cells using Lipofectamine RNAiMax reagent (Thermo Fisher Scientific), according to package instructions. The cells were counted after 3 and 6 days from transfection in triplicate samples using a Nexcelom Bioscience cell counter. Experiments were repeated three times and reported as mean ± SD.

Ambrosini G., Natale C.A., Musi E., Garyantes T, & Schwartz G.K. (2023). The GPER Agonist LNS8801 Induces Mitotic Arrest and Apoptosis in Uveal Melanoma Cells. Cancer Research Communications, 3(4), 540-547.

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LDH Cytotoxicity Assay Protocol

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The collected cell culture supernatants were co-incubated with LDH cytotoxicity assay reaction buffer following the manufacture’s protocol (Clontech, Mountain View, CA). The colorimetric results were quantified using a BioTek microplate reader at 490 nm wavelength. The dead cells were counterstained with 0.4% trypan blue solution and quantified by Nexcelom cell counter.

Zhang G., Luk B.T., Wei X., Campbell G.R., Fang R.H., Zhang L, & Spector S.A. (2019). Selective cell death of latently HIV-infected CD4+ T cells mediated by autosis inducing nanopeptides. Cell Death & Disease, 10(6), 419.

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Cell Viability and Co-culture Protocol

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Cells were resuspended by gentle pipetting and 10 uL aliquots were taken and mixed in a 1:1 ratio with Ao/PI stain for cell counting by Nexcelom Bioscience cell counter. For co-culture experiments, cell number was subtracted from a background using irradiated stromal cells with no MCL cell seeding, which typically amounted to less than 5 percent of total cell number. In none of the experiments was loss of stromal cell number or viability observed with TAK-981 (provided by Takeda Development Center Americas, Inc., Lexington MA) treatment.

Hanel W., Lata P., Youssef Y., Tran H., Tsyba L., Sehgal L., Blaser B.W., Huszar D., Helmig-Mason J., Zhang L., Schrock M.S., Summers M.K., Chan W.K., Prouty A., Mundy-Bosse B.L., Chen-Kiang S., Danilov A.V., Maddocks K., Baiocchi R.A, & Alinari L. (2022). A sumoylation program is essential for maintaining the mitotic fidelity in proliferating mantle cell lymphoma cells. Experimental Hematology & Oncology, 11, 40.

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Isolation of Pleural Exudate Cells

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Pleural exudate cells (PLEC) were obtained through washing the pleural cavity with 10 ml RPMI supplemented with penicillin-Streptomycin (1%) and L-Glutamine (1%). Samples were kept on ice and contaminating erythrocytes were lysed prior to cell counting with a Nexcelom cell counter.

Campbell S.M., Knipper J.A., Ruckerl D., Finlay C.M., Logan N., Minutti C.M., Mack M., Jenkins S.J., Taylor M.D, & Allen J.E. (2018). Myeloid cell recruitment versus local proliferation differentiates susceptibility from resistance to filarial infection. eLife, 7, e30947.

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Lactate Quantification in Cell Culture

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The lactate concentration in the medium was assessed following the protocol provided by the l-lactic acid (LA) Colorimetric Assay Kit (Elabscience, Wuhan, China). LX-2 cells were cultured for 48 h, and the supernatants were collected and diluted with PBS. A 5 µL aliquot of the diluted sample was taken for each well, followed by the addition of 100 µL of enzyme working solution and 20 µL of chromogenic agent. The mixture was then incubated at 37 °C for 10 min. A stop solution was added to each well, and the absorbance was measured at 530 nm using a full-wavelength microplate reader. To account for variations in cell number, the lactate content was normalized using a cell counter (Nexcelom Bioscience, USA). A similar procedure was employed to determine lactate levels in serum.

Zhou Y., Yan J., Huang H., Liu L., Ren L., Hu J., Jiang X., Zheng Y., Xu L., Zhong F, & Li X. (2024). The m6A reader IGF2BP2 regulates glycolytic metabolism and mediates histone lactylation to enhance hepatic stellate cell activation and liver fibrosis. Cell Death & Disease, 15(3), 189.

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Targeted Knockdown of IGF-1R, c-Met, and PDGFRβ

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50 nM siRNAs (pooled siRNAs, Dharmacon) specific for human IGF-1R, c-Met and PDGFRβ were transfected using Lipofectamine RNAiMAX reagent (Invitrogen). Scrambled (non-targeting pool) siRNA was used as control. 48 hours after transfections, cells were trypsinized, counted using a Nexcelom cell counter and plated in 96-well plates for cell viability assays as described. Cells were also collected, lysed and analyzed by western blot to check for knockdown of protein expression.

Patwardhan P.P., Ivy K.S., Musi E., de Stanchina E, & Schwartz G.K. (2015). Significant blockade of multiple receptor tyrosine kinases by MGCD516 (Sitravatinib), a novel small molecule inhibitor, shows potent anti-tumor activity in preclinical models of sarcoma. Oncotarget, 7(4), 4093-4109.

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Isolation and Characterization of Immune Cells from Mouse Spleen and Meninges

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Mice were given a lethal dose of anesthesia by intraperitoneal (i.p.) injection of Euthasol (10% v/v in saline) and were transcardially perfused with ice-cold PBS containing 10 U mL⁻¹ heparin. The spleen and dural meninges were dissected and digested in 1 mg mL⁻¹ collagenase 8, D and 5 U mL⁻¹ DNase I (Sigma Aldrich) at 37°C for 30 min. The digestion was stopped by adding EDTA to a final concentration of 10 mM. The cell suspension was passed through a 70-um cell strainer. Cells were washed with RPMI (Gibco), resuspended in PBS, and counted with a cell counter (Nexcelom). Cells were stained with Zombie Aqua™ dye (Biolegend) for 20 min on ice. Cells were washed with PBS containing 2% FBS (FACS buffer), and Fc-receptors were blocked with anti-CD16/32 antibody in 50 μl total volume. An equal volume of primary antibody mix was added containing anti-CD45.2-Alexa Fluor 700, anti-CD4-eFluor450, anti-CD8a-PE, anti-CD90.2-PE-Cy7, and anti-TCRβ-FITC. Cells were stained for 30 min on ice and washed twice with FACS buffer. The data was acquired on a flow cytometer (Gallios Beckman Coulter) and analyzed using FlowJo software (Tree Star, Inc.) or Cytobank Premium.

Herz J., Fu Z., Kim K., Dykstra T., Wall M., Li H., Salvador A.F., Zou B., Yan N., Blackburn S., Andrews P.H., Goldman D.H., Papadopoulos Z., Smirnov I., Xie X.S, & Kipnis J. (2021). GABAergic neuronal IL-4R mediates T cell effect on memory. Neuron, 109(22), 3609-3618.e9.

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