CRC cell lines (HCT116, SW-620, LOVO, HCT-15 and SW480) and normal epithelial cell lines, CCD-18Co, were procured from the American Type Culture Collection (ATCC). HCT116 cells (cat. no. CCL-247) were maintained in McCoy's 5a medium (cat. no. 30-2007; ATCC); SW-620 (cat. no. CCL-227) and SW480 cells (cat. no. CCL-228) were grown in Leibovitz's L-15 medium (cat. no. 30-2008; ATCC); LOVO cells (cat. no. CCL-229) were maintained in F-12K medium (cat. no. 30-2004; ATCC); HCT-15 cells (cat. no. CCL-225) were cultured in RPMI-1640 medium (cat. no. 30-2001; ATCC); and CCD-18Co cells (cat. no. CRL-1459) were maintained in Eagle's Minimum (cat. no. 30-2003; ATCC). All cells were cultured in media supplemented with 10% FBS (cat. no. 16000044; Gibco; Thermo Fisher Scientific, Inc.) in a humidified containing 5% CO2 at 37°C.
Ccd 18co
Manufactured by American Type Culture Collection
Sourced in United States, Germany
The CCD-18Co is a cell culture dish designed for the propagation and maintenance of cell lines. It is a sterile, disposable plastic dish with a circular growth surface and a tight-fitting lid to maintain a controlled environment. The dish is suitable for a variety of cell culture applications and can be used in incubators to support cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by American Type Culture Collection (72 mentions)
Sw480, by American Type Culture Collection (45 mentions)
Dld 1, by American Type Culture Collection (32 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (30 mentions)
Sw620, by American Type Culture Collection (29 mentions)
Hct15, by American Type Culture Collection (22 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (20 mentions)
Caco 2, by American Type Culture Collection (19 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (14 mentions)
Hct116, by Thermo Fisher Scientific (14 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (13 mentions)
Ccd 18co, by Thermo Fisher Scientific (13 mentions)
Ht 29, by American Type Culture Collection (12 mentions)
Ls174t, by American Type Culture Collection (11 mentions)
Colo205, by American Type Culture Collection (10 mentions)
Ccd 18co, by Merck Group (9 mentions)
Fetal bovine serum (fbs), by Merck Group (8 mentions)
Rpmi 1640, by Thermo Fisher Scientific (7 mentions)
Mcf 7, by American Type Culture Collection (6 mentions)
Penicillin, by Thermo Fisher Scientific (6 mentions)
147 protocols using ccd 18co
1
Culturing Colorectal Cancer Cell Lines
2
Human Colorectal Cell Lines Protocol
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The human CRC cell lines LS174T (CL-188), RKO (CRL-2577), DLD-1 (CCL-221), Caco2 (HTB-37), SW620 (CCL-227), HCT-8 (CCL-244), HCT116 (CCL-247), and HCT-15 (CCL-225); the human normal colorectal epithelial cell line FHC (CRL-1831); and the human normal colorectal fibroblast cell line CCD-18Co (CRL-1459) were all purchased from the American Type Culture Collection (ATCC). All human CRC cells were cultured in RPMI-1640 medium (Gibco, C11875500BT) supplemented with 10% fetal bovine serum (FBS) (ExCell Bio, FND500). FHC was cultured in RPMI-1640 medium supplemented with 15% FBS, and CCD-18Co was cultured in Eagle’s Minimum Essential Medium (EMEM) (ATCC, 30–2003) supplemented with 10% FBS. All cells were cultured at 37 °C in humidified atmosphere containing 5% CO2. Cell line certificates of analysis were obtained from the ATCC. All cell lines were negative for mycoplasma.
3
Colon Cancer Cell Lines and Inhibitors
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CRC cell lines HT-29 and Colo205 and fibroblast cell line CCD-18Co from the colon were purchased from ATCC. Human normal colon mucosal epithelial cell line NCM460 was obtained from INCELL. HT-29 and Colo205 were cultured in complete Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS), with essential supplementation including penicillin/streptomycin solution, while NCM460 in complete RPMI-1640 medium. CCD-18Co was cultured in ATCC-formulated Eagle's Minimum Essential Medium (#30-2003) with 10% FBS. All the cells were incubated at 37°C in a 5% CO2 humidity atmosphere. The recombinant human IL-6 was purchased from R&D Systems. For the function blocking, the specific antibody 10D5 against integrin β6 was purchased from Merck Millipore and neutralizing anti-IL-6 as well as anti-IL-6R from R&D Systems. To knock down integrin β6 (#144659), IL-6R (#106147), or STAT-3 (#116558), cells were transfected with specific siRNA (Thermo Fisher Scientific) using Lipofectamine 3000 (Invitrogen) according to the manufacturer's instructions. For the inhibition of the STAT-3 pathway, specific STAT-3 inhibitors Stattic and Cryptotanshinone (CTS), ERK/MAPK inhibitor U0126, and PI3K inhibitor LY294002 were purchased from SelleckChem.
4
Culturing Human Colon Cell Lines
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Human CRC HCT116, DLD-1, HT29, and SW620 cells, as well as human colon CCD-18Co and FHC cells, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and the HCT116 Luc+ Cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). Moreover, all cell lines were not mycoplasma contamination.
The HCT116 and HCT116 Luc+ cells were grown in McCoy’s 5A medium (Welgene, Farmingdale, NY, USA), the CCD-18Co cells were cultured in Eagle Minimum Essential Medium (EMEM, ATCC), and the other cell lines were grown in RPMI 1640 medium. All media were supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA) in a 37 °C humidified chamber with 5% CO2.
The HCT116 and HCT116 Luc+ cells were grown in McCoy’s 5A medium (Welgene, Farmingdale, NY, USA), the CCD-18Co cells were cultured in Eagle Minimum Essential Medium (EMEM, ATCC), and the other cell lines were grown in RPMI 1640 medium. All media were supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA) in a 37 °C humidified chamber with 5% CO2.
5
Culturing Colorectal Carcinoma and Normal Colon Cells
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Human colorectal carcinoma HCT-116 and fibroblasts from the normal colon (CCD-18Co) were purchased from the American Type Culture Collection (ATCC, MN, United States). HCT-116 cell line was maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, ThermoFisher, Inc., United States) and normal colon cell line CCD-18Co was cultured in Eagle’s Minimum Essential Medium (ATCC, MN, United States). Both media were supplemented with a 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 g/ml streptomycin. Cells were maintained at 37°C in a humidified atmosphere containing 5/95% of CO2/air.
6
Metformin Modulates TGF-β1 in Colon Fibroblasts
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Human colon fibroblast cell line (CCD-18Co) was purchased from American Type Culture Collection (ATCC, Manassas, VA, United States). CCD-18Co cells were cultured in Eagle’s Minimum Essential Medium (EMEM, ATCC) medium supplemented with 10% fetal bovine serum (FBS, ExCell BIO, Shanghai, China) at 37°C, 90% humidity and 5% CO2 incubator. After starving (0% serum) for 24 h, the cells were then treated with 0.1 or 0.5 mM metformin. 4 h later, rhTGF-β1 (2 ng/ml) was added. Untreated cells and cells treated with rhTGF-β1 alone were regarded as controls.
7
Culturing Human Colorectal Cancer Cells
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Human CRC HT29, DLD-1, HCT116, SW480, and SW620, and human normal colon CCD-18Co cells were purchased from American Type Culture Collection (Manassas, VA, USA). Human CRC cells were cultured in RPMI 1640 medium from GenDEPOT (Katy, TX, USA) or McCoy’s 5A medium. CCD-18Co cells were cultured in Eagle’s minimal essential medium (American Type Culture Collection). All media contained 1% antibiotic–antimycotic (100 X; GenDEPOT) and 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA). All cell lines were maintained in a 5% CO2 incubator at 37 °C.
8
Culturing Esophageal and Colon Fibroblasts
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The human esophageal fibroblasts cell lines (FEE4-T and BEF-T) were cultured in Dulbecco’s modified Eagle’s medium and Medium 199 (4:1 ratio) supplemented with 10% fortified bovine calf serum and gentamicin (50 μg/mL). Human colon fibroblast CCD-18Co (ATCC CRL-1459) and human lung fibroblast MRC-5 (ATCC CCL-171) were cultured in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. For individual experiments, fibroblasts were equally seeded, maintained in full growth media, and kept in log phase growth until ready to use. We used two non-neoplastic, telomerase-immortalized, esophageal squamous cell lines (EoE1-T and EoE2-T) that were created by our laboratory using esophageal mucosal biopsy specimens from patients who had EoE, as previously described.[2 (link)] Cells were maintained in monolayer culture at 37°C in humidified air with 5% CO2 in growth medium co-cultured with a fibroblast feeder layer as previously described.[30 (link)] For individual experiments, epithelial cells were equally seeded into standard culture dish without fibroblast feeder cells and maintained in growth medium.
9
Cell Line Culturing for Cancer Research
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Human colon cancer cell lines HCT-116 (ATCC CCL-247) and HT-29 (ATCC HTB-38), the triple negative human breast cancer cell line MDA-MB-231 (ATCC HTB-26), and the normal human colon cell line CCD-18Co (ATCC CRL1459) were obtained from American Type Culture Collection (ATCC) and cultured according to the ATCC indications.
10
Cell Culture of HT-29 and CCD-18Co
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