Female BALB/c nude mice (5–6 weeks old, 20 g) were purchased from ORIENT (Gapyeong, South Korea). The mice were grouped as follows (n = 3 mice per group): (1) PBS as a negative control, (2) NEs, (3) liposomes, and (4) NE-liposomes. The liposomal formulations were injected intravenously (i.v.) by a single treatment, the same as the therapeutic dose. To determine the biochemical toxicity of the liposomal formulations, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and blood urea nitrogen (BUN) were analyzed using diagnostic kits (Roche, Berlin, Germany).27 (link) Blood samples were collected from the mice through the retro-orbital sinus on days 1 and 7 after the injection of liposomes. Serum was obtained from the blood by centrifugation at 3000 rpm at 4 °C for 10 min (Smart R17 Plus, Hanil Scientific Inc., Seoul, South Korea).
Smart r17 plus
Manufactured by Hanil
Sourced in Cameroon
The Smart R17 Plus is a high-performance laboratory centrifuge capable of handling a wide range of sample volumes and types. It features a brushless motor and advanced electronic controls for precise speed and temperature regulation. The centrifuge's robust design and built-in safety features ensure reliable and efficient operation in various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Msh 20a, by Daihan Scientific (2 mentions)
Diagnostic kit, by Roche (1 mentions)
Kontes pellet pestle cordless motor, by Merck Group (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
1 bromo 3 chloropropane, by Merck Group (1 mentions)
Mirneasy mini kit, by Qiagen (1 mentions)
Chemidoc xps imaging system, by Bio-Rad (1 mentions)
Xpr6ud5, by Mettler Toledo (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
S 4700, by Hitachi (1 mentions)
Cary 60, by Agilent Technologies (1 mentions)
6 protocols using smart r17 plus
1
Toxicity Assessment of Liposomal Formulations
2
RNA Extraction Using TRIzol and miRNeasy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted using both TRIzol (Invitrogen, Carlsbad, CA, United States) and the miRNeasy Mini Kit (QIAGEN, Valencia, CA, United States) according to the manufacturers’ protocols, with some modifications. Each sample was resuspended in 300 μl TRIzol reagent (Invitrogen). Resuspension was homogenized for 30 s using a Kontes Pellet Pestle Cordless Motor (Sigma-Aldrich, St. Louis, MO, United States) and placed on ice for 30 s, the steps being repeated 5 times for full homogenization. Then, an additional 700 μl TRIzol was added to the samples and incubated at room temperature for 5 min. 1-bromo-3-chloropropane (200 μl, Sigma-Aldrich) was then added to each sample, followed by incubation for 3 min. Next, the samples were centrifuged at 13,499 × g for 15 min at 4°C using a centrifuge (Smart R17 plus, Hanil Science, Incheon, South Korea). The supernatant (approximately 750 μl) of each sample was mixed with 750 μl of 100% isopropanol. The mixed samples were incubated at -20°C for 2 h. The columns of miRNeasy Mini Kit (QIAGEN) were additionally used to purify the small RNA-enriched RNA and clean RNA according to the manufacturer’s protocol.
3
Targeted Plasma Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For targeted profiling, we added 800 µL of acetonitrile for protein precipitation to 200 µL of each plasma sample, followed by the addition of an internal standard, 1,6-diaminohexane (1 ppm × 20 µL). After protein precipitation, samples were centrifuged at 1200× g for 5 min with Smart R17 plus (Hanil, Kimpo, Korea). The supernatants were transferred to a 10-mL tube to which 100 µL of dansyl chloride (4 mg/mL in acetonitrile) and 100 µL of sodium carbonate buffer (0.1 M, pH 9.0) were added. The mixture was incubated at 60 °C for 15 min. After evaporation, the residue was reconstituted with 100 µL of methanol.
4
Microdevice for Saliva-based Diagnostics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A microcentrifuge (smart R17 plus; Hanil Scientific Inc., Gimpo, Korea) was used to separate the AuNP conjugate, and a multi-purpose centrifuge (1580R; LABOGENE Co., Ltd., Lillerød, Denmark) was used to collect saliva samples. A drying oven (KO-100; LK Lab Co., Namyangju, Korea) was used to dry the membranes and all pads following antibody and sample loading. A dispenser (DCI100; Zeta Co., Gunpo, Korea) was used to immobilize samples on a membrane, and a cutting device (TBC-50Ts; Taewoo Co., Namyangju, Korea) was used to cut the membranes. A field emission scanning electron microscope (Hitachi S-4700, Tokyo, Japan) and a light microscope (Olympus BX43, Tokyo, Japan) were used to characterize the structure of PVA tape. An XPR ultra-microbalance (XPR6UD5; Mettler Toledo, Masstron, OH, USA) was used to weigh tapes. All signals from the strips were measured and analyzed with a ChemiDoc XPS + imaging system and Image lab software (6.1; Bio-Rad Laboratories), respectively. The ELISA results were evaluated using a cell imaging multi-mode reader (Cytation 5).
5
Synthesis and Characterization of HHT-Capped AuNPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To synthesize AuNPs, the optimized conditions were monitored as described earlier.29 (link) HHT extract was dissolved in distilled water at a concentration of 10 mg/mL and passed through a 0.22 µm filter before use. The gold salt (HAuCl4•3H2O) was dissolved in distilled water and used at 100 mM (stock solution). The stock solution of gold salt was added to the HHT solution at different concentrations. The reaction mixtures were incubated in a shaking incubator (MSH-20A, DAIHAN Scientific, Republic of Korea). We optimized the conditions for the synthesis of HHT-AuNPs in terms of HHT and HAuCl4•3H2O concentrations, incubation time, and reaction pH. The synthesis was first monitored by the visual color change of the reaction mixture, followed by spectral analysis. After the complete reduction of the gold salt (1.5 mM) into the nanoparticles, the nanoparticles were collected by centrifugation at 12,000 rpm for 20 min (Smart R17 Plus, Hanil Scientific Inc., Republic of Korea). The obtained nanoparticles were washed thoroughly with distilled water to remove unconverted metal ions or other constituents. Finally, the nanoparticles were collected by air-drying and used for analytical characterization and application.
6
Biosynthesis of CJ-Derived Gold Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To prepare CJ-AuNPs, the optimized conditions were monitored in the previous study21. CJ ethanol extract was dissolved in distilled water and passed through a 0.22 µm filter (Advantec) before use. The filtered CJ was mixed with HAuCl4·3H2O solution at different concentrations. The reaction mixture was incubated in a shaking incubator (MSH-20A; Daihan Scientific, Gangwon-do, Korea). The optimized conditions for the CJ-AuNPs biosynthesis were explored in terms of concentrations of CJ ethanol extract and HAuCl4·3H2O, incubation time, and reaction pH value. The completion of biosynthesis was decided by visualization of changed color (from yellow to purple), following determination of optical density using UV-visible (Vis) spectrophotometer (Cary 60; Agilent Technologies, Santa Clara, CA, USA). To remove the soluble materials, the mixture was centrifuged (12,000 rpm, 20 min) using a centrifuge (Smart R17 Plus, Hanil Scientific Inc., Korea), and the precipitated particles were washed with distilled water. This process was repeated four more times to purify the particles. Finally, the CJ-AuNPs were stored at 4 °C for further cell treatment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!