A549 cells, H1299 cells, and H460 cells were purchased from ATCC. The cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere at 5% CO2, and treated with PF-573228 (TOCRIS, Bristol, UK) at concentrations of 0, 0.1, 1, or 10 μM.
H460 cells
Manufactured by American Type Culture Collection
Sourced in United States
H460 cells are a human lung cancer cell line derived from a large cell carcinoma. They are commonly used in research for studying lung cancer biology and for evaluating potential therapeutic interventions.
Automatically generated - may contain errors
Lab products found in correlation
A549 cell, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Hek293 cell, by American Type Culture Collection (2 mentions)
H1299 cells, by American Type Culture Collection (1 mentions)
Pf573228, by Bio-Techne (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Egm 2 singlequot, by Lonza (1 mentions)
Huvecs, by Thermo Fisher Scientific (1 mentions)
Ampfistr identifiler pcr amplification kit, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Llc cells, by American Type Culture Collection (1 mentions)
12 protocols using h460 cells
1
PF-573228 Cytotoxicity Assay
2
Fluorescent Imaging of KRAS Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorescent imaging was carried out of TO-PNA-peptides
in KRAS wild type and mutant cells. SK-LU-1 cells
(ATCC) were cultured in EMEM medium with 10% fetal bovine serum (FBS).
H460 cells (ATCC) were cultured in RPMI 1640 medium with 10% FBS.
20 000 cells were seeded in an 8-chamber well slide (Millipore)
in 10% growth medium and incubated overnight. 200 nM TO-PNA-peptides
were added to each well in serum-free medium and were incubated for
4 h at 37 °C to allow equilibration of bound TO-PNA-peptide.
At the end of incubation, cells were washed 3× with PBS containing
Ca2+ and Mg2+ (Fisher) followed by fixation
with 4% paraformaldehyde in PBS. Cells were then washed one more time
with PBS containing Ca2+ and Mg2+. Chambers
were removed and the slides were mounted with Prolong Gold Antifade
reagent with DAPI (Life Technologies). All images were taken on a
Nikon C1 Plus two point-scanning laser confocal microscope using 40×
oil objective. Excitation was at 488 nm, and emission was observed
at 535/40 nm.
in KRAS wild type and mutant cells. SK-LU-1 cells
(ATCC) were cultured in EMEM medium with 10% fetal bovine serum (FBS).
H460 cells (ATCC) were cultured in RPMI 1640 medium with 10% FBS.
20 000 cells were seeded in an 8-chamber well slide (Millipore)
in 10% growth medium and incubated overnight. 200 nM TO-PNA-peptides
were added to each well in serum-free medium and were incubated for
4 h at 37 °C to allow equilibration of bound TO-PNA-peptide.
At the end of incubation, cells were washed 3× with PBS containing
Ca2+ and Mg2+ (Fisher) followed by fixation
with 4% paraformaldehyde in PBS. Cells were then washed one more time
with PBS containing Ca2+ and Mg2+. Chambers
were removed and the slides were mounted with Prolong Gold Antifade
reagent with DAPI (Life Technologies). All images were taken on a
Nikon C1 Plus two point-scanning laser confocal microscope using 40×
oil objective. Excitation was at 488 nm, and emission was observed
at 535/40 nm.
3
Culturing HeLa and H460 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa cells and H460 cells (ATCC, USA) were grown in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 µg/ml penicillin and 100 U/ml streptomycin). Cells were incubated at 37oC in a 5% CO2/95% humidity atmosphere. All the cells used in the experiments were passaged at numbers less than 12.
4
Cell Culture Protocols for HEK293, WI-38, and H460
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293 cells (ATCC, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium (Gibco BRL, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco BRL), and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). WI-38 cells (Lonza, Basel, Switzerland) were maintained in Dulbecco’s modified Eagle’s medium (Gibco BRL, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco BRL), 1% MEM Non-Essential Amino Acids (Gibco BRL) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA). H460 cells (ATCC, Manassas, VA, USA) and H460 stable cell lines were maintained in RPMI 1640 medium (Gibco BRL) supplemented with 10% fetal bovine serum (Gibco BRL) and 1% penicillin/streptomycin (Invitrogen). All cell lines were incubated at 37 °C with 5% CO2.
5
Lung Cancer Cell Culture in RPMI 1640
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lung cancer H460 cells were obtained from the American Type Culture Collection (Manassas, VA). Cells were cultured in RPMI 1640 containing 5% fetal bovine serum, 2 mM l -glutamine, and 100 units/mL penicillin/streptomycin in a 5% CO2 environment at 37°C. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and other chemicals were obtained from Sigma Chemical, Inc. (St. Louis, MO).
6
Characterization of Cell Lines for Cytotoxicity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human cervical carcinoma HeLa and laryngeal carcinoma HEp-2 cells were obtained from cell culture bank (GIBCO BRL, Invitrogen, Grand Island, NY, USA). HEp-2 cell line was recently recognized and categorized by ATCC as human laryngeal carcinoma cell line cross-contaminated with HeLa cells. The development of HEp-2 subline resistant to carboplatin (7T) has been published previously.19 (link) These cells are cross-resistant to anticancer drug cisplatin, transplatin, mitomycin C and the natural compound curcumin as well.19 (link)-21 (link) Large cell lung carcinoma H460 cells and colorectal carcinoma HCT-116 were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). Normal human skin fibroblasts were isolated from the upper arm of a 7-years-old female donor at the Neurochemical Laboratory, Department of Chemistry and Biochemistry, School of Medicine, University of Zagreb. They were used for the cytotoxicity assay at 32 and 36 population doublings. All cell lines were grown as a monolayer culture in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich) in a humidified atmosphere of 5% CO2 at 37°C and were sub-cultured every 3 – 4 days.
7
Cell Culture Protocol for H460 and H226B
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H460 cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). H226B cells were kindly provided by Jack A. Roth (MD Anderson Cancer Center, Houston, TX, USA). Cells were grown at 37°C in a humidified incubator under 5% CO2 in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and antibiotics (all from WelGENE). Cells were sub-cultured every 2-3 days at approximately 80–90% confluency.
8
Culturing Human and Murine Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human NSCLC line (H460 cells) and a murine lung cancer cell line (LLC cells) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Human umbilical vein endothelial cells (HUVECs) were purchased from Thermo Fisher Scientific, Waltham, MA, USA). The cells were cultured in RPMI 1640 medium (H460 cells) or DMEM (for LLC cells) supplemented with 10% fetal bovine serum (FBS) and antibiotics (all from WelGENE, Kyeongsan-si, Republic of Korea) and maintained at 37°C with 5% CO2 in a humidified atmosphere. The HUVECs were cultured in endothelial cell basal medium [EBM-2 (Lonza Inc., Allendale, NJ, USA)] supplemented with EGM-2 SingleQuots (Lonza). HUVECs between passages 3 and 8 were used. Chemotherapy-resistant cells were generated by continuous exposure to increasing concentrations of corresponding chemotherapeutic drugs for more than 6 months. NSCLC cells and their chemotherapy-resistant subpopulations were authenticated and validated using the AmpFISTR Identifiler PCR Amplification Kit (Applied Biosystems, Foster, CA; cat. No. 4322288) in 2013, 2016, and 2017. Cells passaged for fewer than 6 months after receipt and resuscitated validated cells were used in this study.
9
Sirt1 Regulates EMT in Lung Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hainan Asia Pharmaceutical Co., Ltd. (Haikou, China) provided PPD, which had a purity of > 95% as determined by high-performance liquid chromatography. A549 cells and H460 cells were obtained from American Type Culture Collection (Manassas, VA, United States) and cultured at 37°C in a humidified atmosphere of 5% CO2 with RPMI-1640 medium, which was supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 mg/mL streptomycin. Ang II and EX-527 were purchased from Sigma-Aldrich (St. Louis, MO, United States). The BCA protein assay reagent kit and DAPI staining kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). The primary antibodies for E-cadherin, vimentin, and SIRT1 were purchased from Abcam (Cambridge, United Kingdom). Slug and ZEB1 were purchased from Cell Signaling Technology (Danvers, MA, United States). GAPDH was purchased from ZSGB Biotechnology Co., Ltd. (Beijing, China). The secondary antibodies were purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd. (Beijing, China).
10
Culturing H460 Lung Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H460 cells (from human non-small cell lung carcinoma: NSCLC) were purchased from the American Type Culture Collection (Manassas, Virginia, USA) and cultured in RPMI-1640 medium supplemented with L-glutamine, antibiotic/antimycotic solution (Sigma Chemical Co., St. Louis, MO, USA), and with 10% fetal bovine serum (FBS). Cells were incubated at 37 C in a 5% CO 2 atmosphere.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!