Reboxetine mesylate

Traxoprodil (5, 10, 20, and 40 mg/kg, Sigma-Aldrich) was suspended in a 1 % aqueous solution of Tween 80 (POCH), whereas imipramine hydrochloride (15 and 30 mg/kg, Sigma-Aldrich), fluoxetine hydrochloride (5 mg/kg, Sigma-Aldrich), escitalopram oxalate (2 mg/kg, Sigma-Aldrich), reboxetine mesylate (2.5 mg/kg, Abcam Biochemicals), WAY 100,635 (0.1 mg/kg, Sigma-Aldrich), and ritanserin (4 mg/kg, Sigma-Aldrich) were dissolved in physiological saline (0.9 % NaCl). The solutions/suspension were prepared immediately prior to the experiments and were administered intraperitoneally (i.p.) 60 min before testing. The doses and pretreatment schedules were selected on the basis of the literature data and the results of our previous experiments (Poleszak et al. 2005 (link), 2007a (link), 2011 (link), 2013 (link); Szewczyk et al. 2002 (link), 2009 (link)). Animals from the control groups received i.p. injections of the vehicle (saline). The volume of all administered solutions/suspension was 10 ml/kg.

Caffeine (1,3,7-trimethylxanthine; 5, 10, 20, and 50 mg/kg, Sigma-Aldrich, Poznań, Poland), imipramine hydrochloride (15 and 30 mg/kg, Sigma-Aldrich), desipramine hydrochloride (10 mg/kg, Sigma-Aldrich), fluoxetine hydrochloride (5 mg/kg, Sigma-Aldrich), escitalopram oxalate (2 mg/kg, Sigma-Aldrich), paroxetine hydrochloride (0.5 mg/kg, Abcam Biochemicals, Cambridge, UK), and reboxetine mesylate (2.5 mg/kg, Abcam Biochemicals) were dissolved in 0.9 % NaCl. The solutions of antidepressants were administered intraperitoneally (i.p.) 60 min before behavioral testing, whereas Caffeine solution was administered i.p. 40 min before the tests. The doses and pretreatment schedules were selected on those reported in the literature and on the basis of the results of our previous experiments (David et al. 2003 (link); Poleszak et al. 2006 (link); Szewczyk et al. 2002 (link); Szewczyk et al. 2009 (link); Urani et al. 2001 (link)). All solutions were prepared immediately prior to the experiment. Animals from the control groups received i.p. injections of the vehicle (0.9 % saline). The volume of the vehicle or drug solutions for i.p. administration was 10 ml/kg.

The following drugs were used: benserazide hydrochloride, 3,4-dihydroxy-l-phenylalanine (L-DOPA), 6-hydroxydopamine hydrochloride (6-OHDA) and desipramine hydrochloride (Sigma-Aldrich, St. Louis, MO, USA), pramipexole dihydrochloride (Tocris, Bristol, UK) and reboxetine mesylate (Abcam Biochemicals, Cambridge, UK). 6-OHDA was dissolved in saline containing 0.02% ascorbic acid and locally injected in the brain. Desipramine (25 mg/kg, i.p.), pramipexole (0.6 mg/kg, s.c.), reboxetine (20 mg/kg, i.p.) and L-DOPA [10 mg/kg, i.p.; administered together with the peripheral DOPA decarboxylase inhibitor, benserazide (7.5 mg/kg)] were dissolved in saline. All drugs were injected in a volume of 10 ml/kg. Desipramine was administered 30 min before the injection of 6-OHDA. The other drugs were administered for four consecutive days preceding the experiment. The animals were tested 30 min after the last drug administration.