E el h5812c

CXCL12 (E-EL-H0052c, ElabScience, Wuhan, China), IL17A (E-EL-H5812c, ElabScience), MPO (ab119605, Abcam), and MMP-9 (E-EL-H6075, ElabScience) levels in blood plasma samples were measured using ELISA kits according to the manufacturer’s instructions. In brief, samples or standards were added to the wells and incubated for 90 min at room temperature. After washing, a working detector was added to each well, followed by the addition of the substrate solution. The reaction was stopped, and the absorbance was read at 450 nm. The amount was calculated using a standard curve and GraphPad Prism 9.5.0.

The serum concentrations of HMGB1, TLR4, IL‐23, and IL‐17A of psoriasis patients and healthy controls were measured by ELISA according to the manufacturer's instructions for three repeated experiments (cat. no E‐EL‐H1554C, E‐EL‐H5820C, E‐EL‐H0107C, and E‐El‐H5812C; Wuhan Elabscience Biotechnology).