Ewing sarcoma cell lines (n = 21) were obtained from multiple sources: L-1062 and L-872 were established in-house [21 (link)]; CHP100, RM-82, IARC-EW7, TC32 and 6647, CHP100, RM-82, IARC-EW-7, WE-68, IARC-EW-3, STA-ET-2.1, TTC-466, STA-ET-10, CADO-ES1, TC-71, VH-64, COH and STA-ET-1 were obtained from the EuroBoNeT consortium collection (Institute of Pathology, University Medical Center, Düsseldorf, Germany) [22 (link)] and SK-ES-1, SK-NM-C, A-673 and R-D-ES from the American Type Culture Collection (ATCC). All cell lines and primary culture L-4027 were cultured in a monolayer under equal conditions and in Iscove’s modified Dulbecco’s medium containing GlutaMAX supplement, supplemented with 1 % streptomycin/penicillin and 10 % heat-inactivated FCS (all from Life Technologies, Bleiswijk, The Netherlands). Authentication of cell lines using Powerplex 1.2 and CellID STR (Promega, Leiden, the Netherlands) and mycoplasma DNA Q-PCR screening were regularly performed on all cell lines.
Cellid str
Manufactured by Promega
CellID STR is a short tandem repeat (STR) analysis kit designed for cell line identification. It enables the analysis of multiple STR loci to generate a unique genetic profile for cell lines, allowing for the verification of cell line identity.
Automatically generated - may contain errors
Lab products found in correlation
Powerplex 1, by Promega (2 mentions)
Glutamax supplement, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (1 mentions)
Sk n mc, by American Type Culture Collection (1 mentions)
Rd es, by American Type Culture Collection (1 mentions)
Sk es 1, by American Type Culture Collection (1 mentions)
We 68, by American Type Culture Collection (1 mentions)
Ttc 466, by American Type Culture Collection (1 mentions)
Vh 64, by American Type Culture Collection (1 mentions)
3 protocols using cellid str
1
Ewing Sarcoma Cell Lines Comprehensive Characterization
2
Comprehensive Cell Line Culture Protocol
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Twenty-three EWS cell lines and one primary culture were cultured as described by Sand et al. [16] (link). Human embryonic kidney cell line HEK293T was cultured in DMEM with 10% FCS without antibiotics; human T cell leukemia cell line Jurkat, colon carcinoma cell lines SW480, HT29 and HCT116; cervical carcinoma cell lines Hela, Siha and Caski; breast carcinoma cell lines MDA-MB-231 and MCF7 were obtained from ATCCfoot_0 and cultured in RPMI with 10% heat inactivated FCS without antibiotics. All cell lines were regularly tested on mycoplasma and authentication with DNA Q-PCR screening and CellID STR, respectively (Promega, Leiden, The Netherlands).
3
Authentication of Ewing Sarcoma Cell Lines
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21 Ewing sarcoma cell lines were obtained from multiple sources: L-1062 and L-872 were established 1: event reported or 0: no event reported. c 1 tumour volume > 200 ml or 0: < 200 ml. h 1: < 10% tumour vitality or 0 > 10% tumour vitality. [22] and authentication of cell lines using Powerplex 1.2 and CellID STR (Promega, Leiden, The Netherlands) were performed on all cell lines.
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