Neu1 plasmid dna

Bovine serum albumin (BSA), anti-phosphotyrosine (cat no-4G10), anti-phospho-IRF3 (cat no-SAB4504031) and anti-IRF3 (cat no-SAB3500280) antibodies were from Sigma (St.Louis, MO). Neuraminidase from Arthrobacter ureafacians (10269611001) was obtained from Roche (Germany). RNeasy Mini Kit was from Qiagen (Limburg, Netherlands); Reverse Transcriptase Kit was from Promega (USA). DyNAmo Color Flash SYBR Green qPCR kit was procured from Thermo Scientific (Rockford, IL). Antibodies-Neu1 (cat no PA5-42552) and Triad3A (cat no-PA5-20079) was from Thermo Fisher Scientific (Rockford, USA). Anti-TLR4 (cat no- MTS510), anti-siglec-E (cat no-377477) antibodies and siglec-E si RNA (cat no-sc-153462) was from Santa Cruz Biotechnology. Anti-Myd88 (cat no-NBP2-27369) and anti-TRIF (cat no-NB120-13810SS) was from Novus Biologicals (Littleton, USA). The cytokine ELISA kits (IFN-β, TNF-α and IL-6) were from Elabscience and from BD-Biosciences (IFN-γ and IL-12). Neu1 plasmid DNA was obtained from Origene (MR226903). All other antibodies were from Cell signaling Technologies (Danvers, MA) unless indicated otherwise. Giemsa stain was obtained from Himedia (India).

Fluorescein isothiocyanate (FITC), bovine serum albumin (BSA), 4′, 6-diamidino-2-phenylindole (DAPI), Giemsa stain, and 2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NeuAc), 4-methylumbelliferone (MU) were from Sigma (St. Louis, MO). Mounting medium was from Amersham Biosciences (Uppsala, Sweden); Maackia amurensis lectin II (MALII) and Sambucu snigra lectin (SNA) were from Vector Labs, and DyNAmo Color Flash SYBR Green qPCR kit was from Thermo Scientific (Rockford, IL). Anti-Neu1, cathepsin A was from Invitrogen (Carlsbad, CA), Anti-TLR4 antibody was from Santa Cruz Biotechnology (MTS510). Anti-Myd88 was from R&D Systems (MN, USA). Anti-phosphotyrosine antibody was from Biolegend (San Diego, CA). All the cytokine ELISA kits were from BD pharmingen, Neu1 plasmid DNA was from Origene (MR1049), Neu1 shRNA was obtained from Sigma (SHCLNG-NM010893), RNeasy Mini Kit was from Qiagen (Limburg, Netherlands); Reverse Transcriptase Kit was from Promega (WI, USA). All other antibodies were from Cell Signaling Technologies (Danvers, MA) unless indicated otherwise.