Mts celltiter 96

Example 6

Using RT-PCR analysis of twenty B-CLL with Rai stages ranging from 0-4, fifteen of twenty patients show elevated HDAC6 expression (FIG. 1A). Viability of CLL cell line Mec1 in this experiment was determined using MTS CellTiter 96® (Promega) analysis, and the results are shown in FIG. 1B. Two polyclonal HDAC6KD cells were developed by stably transfecting them with HDAC6 shRNA (Sigma-Plasmid NM_006044), and subsequently analyzed for viability and compared to non-targeted control counterpart (FIG. 1B—Left part). Mec1 cells were transfected with increasing concentrations of HDAC6 over-expressing (H6OE) plasmid following viability analysis. Next, Mec1 cells were treated with two HDAC6 inhibitors at various doses and assessed for viability by MTS (FIG. 1B—Right part).