Mouse (strain C57BL/6J) spleens were disrupted in PBS containing 2% FBS. Splenic lymphocytes were isolated using EasySep T cell or B cell Isolation Kits (Cat#: P19851, Cat#: 19854, Stemcell Technologies, Vancouver, Canada). Above isolated lymphocytes were stained with anti-CD4, CD8a or CD45R antibodies combined and mixed with Annexin V binding buffer. The samples were acquired on the LSRII flow cytometer (BD Biosciences, Ml, USA) and the data analyzed with Flowjo software (Tree Star, OR, USA). FITC Annexin V Apoptosis Detection Kit with Propidium Iodide (PI) (Cat#: 640914, BioLegend, CA, USA) was employed to detect apoptotic cell Jurkat cells. The samples were analyzed through BD Accuri C6 flow cytometer (BD Biosciences, MI, USA).
Easysep t cell or b cell isolation kits
Manufactured by STEMCELL
Sourced in Canada
The EasySep T cell or B cell Isolation Kits are magnetic-based cell separation products designed for the isolation of human or mouse T cells or B cells from a variety of sample types. The kits use antibody-coated magnetic particles to label and subsequently isolate the target cell population, allowing for the rapid and gentle separation of the desired cells from the starting sample.
Automatically generated - may contain errors
2 protocols using easysep t cell or b cell isolation kits
1
Apoptosis Analysis of Mouse Splenic Lymphocytes
2
Isolation and Apoptosis Analysis of Immune Cells
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Mouse (strain C57BL/6J) spleens were disrupted in PBS containing 2% fetal bovine serum (FBS). Splenic lymphocytes were isolated using EasySep T cell or B cell Isolation Kits (cat# P19851, cat# 19854; Stemcell Technologies, Vancouver, Canada). The aforementioned isolated lymphocytes were stained with anti-CD4, CD8a or CD45R antibodies combined and mixed with annexin V binding buffer. The samples were acquired on the LSRII flow cytometer (BD Biosciences, Michigan, USA), and the data were analysed with FlowJo software (Tree Star, Oregon, USA). FITC Annexin V Apoptosis Detection Kit with propidium iodide (cat# 640914; BioLegend, California, USA) was employed to detect apoptotic cells in Jurkat cells. The samples were analysed through BD Accuri C6 flow cytometer (BD Biosciences).
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