Rho activator 2 cn03

Manufactured by Cytoskeleton

Rho Activator II (CN03) is a laboratory reagent that activates the Rho protein. The Rho protein plays a crucial role in cellular processes such as cytoskeleton organization, cell adhesion, and cell motility. Rho Activator II (CN03) can be used in cell-based assays and biochemical experiments to study the function and regulation of the Rho protein.

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Lab products found in correlation

Y 27632, by Cytoskeleton (1 mentions) α tubulin, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Geldanamycin, by Merck Group (1 mentions) Exoenzyme c3 transferase, by Cytoskeleton (1 mentions) Human pth 1 34, by Bachem (1 mentions) Human pth 1 31, by Bachem (1 mentions) Phalloidin cf405, by Biotium (1 mentions) Ezrin, by Abcam (1 mentions) Ab86297, by Abcam (1 mentions) Pkcζ sc 216, by Santa Cruz Biotechnology (1 mentions) Cycloheximide (chx), by MP Biomedicals (1 mentions) Gm130, by BD (1 mentions) Mabt11, by Merck Group (1 mentions) Pmlc 2, by Abcam (1 mentions)

3 protocols using rho activator 2 cn03

Rho Signaling Pathway Regulation

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BT549 and Hs578T cells were cultured in DMEM (Corning Cellgro) with 10% fetal bovine serum and 1% Penicillin/Streptomycin and maintained at 37°C in 5% CO₂. Y-27632 and Rho Activator II (CN03) were obtained from Cytoskeleton, Inc. The Rho Activator II drug concentration used for this study was determined based on the dose at which maximum stress fiber formation was observed, as recommended by the manufacturer. Primary antibodies used for immunoblotting and/or immunofluorescence were obtained from Cell Signaling Technology for P-MYPT1 (T853), Total-MYPT1, P-MLC (S19), Total-MLC, P-cofilin (S3), Total-cofilin and Acetyl (L-40) tubulin; Abcam for P-tau (S262) and detyrosinated-tubulin; and alpha-tubulin was obtained from Sigma.

Bhandary L., Whipple R.A., Vitolo M.I., Charpentier M.S., Boggs A.E., Chakrabarti K.R., Thompson K.N, & Martin S.S. (2015). ROCK inhibition promotes microtentacles that enhance reattachment of breast cancer cells. Oncotarget, 6(8), 6251-6266.

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Investigating Receptor Signaling Pathways

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Human PTH(1–34), human PTH(1–31), and bovine PTH(3–34) were purchased from Bachem, reconstituted at 100 µM stock, aliquoted and frozen at −80 °C. The Rho inhibitor exoenzyme C3 transferase and the Rho Activator II (CN-03) were each purchased from Cytoskeleton. 8-bromo-cAMP was from Calbiochem, and geldanamycin was from Sigma.

Wong A., Loots G.G., Yellowley C.E., Dosé A.C, & Genetos D.C. (2015). Parathyroid hormone regulation of hypoxia-inducible factor signaling in osteoblastic cells. Bone, 81, 97-103.

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Antibodies and Reagents for Cell Biology

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The following antibodies were used in this study: GM130 (mouse; 610822; BD Transduction Laboratories), DPPIV (CD26, clone OX-61; mouse; MCA924R: Bio-Rad), ezrin (rabbit; ab41672, Abcam), P-MLC2 (Phospho-Myosin Light Chain 2; rabbit; 3674; Cell Signaling), PKC ζ (sc-216; Santa Cruz Biotechnology, Inc.), TGN38 (provided by D. Shields, Albert Einstein College of Medicine, New York), RhoA (rabbit; ab86297; Abcam), VSVG cytoplasmic domain (clone P4D5; hybridoma provided by E. Rodriguez-Boulan, Weill Cornell Medical College, New York), and ZO-1 (rat; MABT11; EMD Millipore). Rho activator II (CN03) was from Cytoskeleton (Denver, Colorado). Cycloheximide was from MP Biomedicals (Santa Ana, California) and AP21988 was from ARIAD Pharmaceuticals (Cambridge, Massachusetts). Phalloidin-CF405 and -Atto 647 N were purchased from Biotium (Fremont, California) and Sigma (St. Louis, Missouri), respectively. SNAP-Cell TMR-Star (TMR*) substrate was from New England BioLabs (Ipswich, Massachusetts).

Lázaro-Diéguez F, & Müsch A. (2020). Low Rho activity in hepatocytes prevents apical from basolateral cargo separation during trans-Golgi network to surface transport. Traffic (Copenhagen, Denmark), 21(5), 364-374.

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