Samples from patients with sepsis and controls were thawed and analyzed in parallel using the LEGENDplex Human Inflammation for IFN-α2, IFN-γ, IL-1β, IL-6, IL-10, TNFα, and IL-18; the LEGENDplex Human Hematopoietic Stem Cell Panels for M-CSF and GM-CSF; and the LEGENDplex Human Vascular Inflammation Panel for S100A8/9 (BioLegend). Flow cytometry data were acquired on a CytoFLEX LX (Beckman Coulter) and analyzed using FlowJo v10.1.
Samples from patients with COVID-19 and controls were analyzed using a commercially available multiplexed proximity extension assay (Olink Proteomics) as detailed by Filbin et al. (77 ). Normalized protein expression (NPX) values are calculated from the number of mapped counts on a NovaSeq run (Illumina) for each protein and represent a relative quantification scale (log2 fold change over the mean of all proteins across all plasma samples).
Samples from patients with COVID-19 and controls were analyzed using a commercially available multiplexed proximity extension assay (Olink Proteomics) as detailed by Filbin et al. (77 ). Normalized protein expression (NPX) values are calculated from the number of mapped counts on a NovaSeq run (Illumina) for each protein and represent a relative quantification scale (log2 fold change over the mean of all proteins across all plasma samples).