Ten thousand PTENP1 or Mock tumour cells were suspended in 400 μl of the appropriate medium supplemented with 2% FBS and were seeded into the upper compartments of a 24-well transwell system with 8-μm pore size polycarbonate filters (Millipore, Billerica, MA, USA). The lower compartments contained 900 μl of medium with 10% FBS. On the second day of incubation, the upper transwells were stained with 0.25% crystal violet. The inner sides of the transwells were scrubbed away and the outer sides were photographed. The crystal violet was washed with 100 μl of 33% acetic acid. The absorbance values of the liquid were detected with a microplate reader at 630 nm.
8 μm pore size polycarbonate filters
Manufactured by Merck Group
Sourced in United States
The 8-μm pore size polycarbonate filters are a type of laboratory equipment designed to separate and filter particles from liquids. These filters have a pore size of 8 micrometers, which allows them to effectively capture particles larger than this size while allowing smaller particles and liquids to pass through.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 8 μm pore size polycarbonate filters
1
PTENP1 Cell Migration Assay
2
Transwell Migration Assay for Bone Marrow-Derived Macrophages
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BMDMs isolated from WT and TGR5−/− mice were cultured and differentiated for 7
days, and then stimulated with LPS for 24 h. The supernatant was collected as conditioned
medium and stored at −80°C. For cell migration assay, 1 × 104 WT BMDMs in 400
μl were seeded in the upper chambers of transwell units with 8-μm pore size polycarbonate
filters (Millipore, Bedford, MA, USA) in serum-free medium, and 600 μl of the different
conditioned media were added to the lower chambers. After incubation for 24 h, cells that
did not migrate through the pores and remained in the upper chamber were removed by
scraping the membrane with a cotton swab. The filters were also stained with 0.1% crystal
violet for 30 min. The numbers of cells that migrated from the upper to the lower surface
of the filter were counted and analyzed using a digital microscope system (Leica, Wetzlar,
Germany).
days, and then stimulated with LPS for 24 h. The supernatant was collected as conditioned
medium and stored at −80°C. For cell migration assay, 1 × 104 WT BMDMs in 400
μl were seeded in the upper chambers of transwell units with 8-μm pore size polycarbonate
filters (Millipore, Bedford, MA, USA) in serum-free medium, and 600 μl of the different
conditioned media were added to the lower chambers. After incubation for 24 h, cells that
did not migrate through the pores and remained in the upper chamber were removed by
scraping the membrane with a cotton swab. The filters were also stained with 0.1% crystal
violet for 30 min. The numbers of cells that migrated from the upper to the lower surface
of the filter were counted and analyzed using a digital microscope system (Leica, Wetzlar,
Germany).
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