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3 protocols using mmp 2 ab92536

1

Molecular Mechanisms of Prostanoid Signaling

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TQ (purity 99%), gelatin, eosin Y, hematoxylin, and β-actin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). EP2 antibody (#101750), PGE2, butaprost, and were purchased from Cayman Chemical (Ann Arbor, MI, USA). SB203580 was obtained from Calbiochem (San Diego, CA, USA). LY294002, Akt (#9272), p-Akt (#9271), p38 (#9212), and anti-rabbit IgG horseradish peroxidase-conjugated antibodies were obtained from Cell Signaling Technologies (Beverly, MA, USA). MMP-2 (ab92536) and MMP-9 (ab76003) antibodies were purchased from Abcam (Cambridge, UK). The p-p38 antibody (#sc-17852) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA).
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2

Fisetin Modulates Signaling Pathways

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Fisetin was purchased from Sigma (St. Louis, MO). EGF was purchased from R&D Systems, Inc (Minneapolis, MN). Antibodies against p-AKT (Ser 473; sc-7985-R), t-AKT (sc-56878), NF-κB (sc-372), c-fos (sc-52), Sp1, Lamin B (sc-6216), and β-actin (sc-47778) were purchased from Santa Cruz Biotechnology (Dallas, TX). MMP-2 (ab92536) and MMP-9 (ab137867) were purchased from Abcam (Cambridge, UK). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma. LY294002 was purchased from Calbiochem (San Diego, CA).
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3

Evaluating MATK Signaling in Cancer

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The reagents and antibodies purchased include: Dulbecco's modified Eagle's medium (DMEM), Corning; fetal bovine serum (FBS), Krackler; Src (2109 T), phospho‐Src Family (Tyr416) (6943 T), phospho‐Src (Tyr527) (2107 T), CHK/MATK (20729S), Csk (4980 T), beta‐Actin (4970 T), GAPDH (97 166 T), Cell Signaling Technology; MMP‐2 (ab92536), Abcam; Doxorubicin hydrochloride (D1515‐916), Sigma; Lenti ORF particles, MATK (mGFP‐tagged)‐ Human megakaryocyte‐associated tyrosine kinase (MATK) transcript variant 1, Origene.
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