Cell surface marker expression was analyzed on a Becton Dickinson LSR FortessaTM flow cytometer after staining with fluorochrome-conjugated antibodies. Intracellular staining was performed using the fix/perm buffer set (Biolegend), according to the manufacturer's instructions. Antibodies used were conjugated anti-NKp30 (P30–15, Biolegend), anti-NKp44 (P44–8.1, BD), anti-NKp46 (29A1.4, Biolegend), anti-IFN-γ (485.B3, Biolegend), anti-TNF-α (MAb11, eBioscience), anti-RANKL (MIH24, Biolegend), anti-Ki-67 (16A8, Biolegend), anti-CD107a (H4A3, BD), anti-CD57 (HCD57, Biolegend), anti-CD56 (N901, Beckman Coulter), anti-CD14 (HCD14, Biolegend), anti-CD16 (3G8, Biolegend), and anti-CD3 (UCHT1, Biolegend). Annexin V/7-AAD staining was performed according to the manufacturer's protocol (Biolegend). The data were analyzed using FlowJo v10.2 software.
Anti nkp44 p44 8
Manufactured by BD
The Anti-NKp44 (P44–8.1) is a laboratory reagent used for the identification and characterization of NKp44-positive cells. It is a monoclonal antibody that specifically binds to the NKp44 receptor expressed on the surface of natural killer (NK) cells and a subset of T cells. The antibody can be used in various immunological techniques, such as flow cytometry, to detect and quantify NKp44-positive cell populations.
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Lab products found in correlation
Anti cd3 ucht1, by BioLegend (2 mentions)
Anti ifn γ 485 b3, by BioLegend (2 mentions)
Anti cd56 n901, by Beckman Coulter (2 mentions)
Anti nkp30 p30 15, by BioLegend (2 mentions)
Lsrfortessa flow cytometer, by BD (2 mentions)
Annexin v 7aad, by BioLegend (1 mentions)
Anti cd107a h4a3, by BD (1 mentions)
Anti tnf α mab11, by Thermo Fisher Scientific (1 mentions)
Fix perm buffer set, by BioLegend (1 mentions)
Anti nkp46 29a1, by BioLegend (1 mentions)
Anti ki 67 16a8, by BioLegend (1 mentions)
2 protocols using anti nkp44 p44 8
1
Multiparametric Flow Cytometry Analysis
2
Multiparametric Flow Cytometry Immunophenotyping
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Cells were analyzed using a BD LSR Fortessa™ flow cytometer following staining with fluorochrome conjugated antibodies. Prior to staining, cells were fixed using the fix buffer set (Biolegend), according to the manufacturer’s instructions. Antibodies used within this study were conjugated anti-CD3 (UCHT1, Biolegend), anti-CD56 (N901, Beckman Coulter), anti-NKp30 (P30-15, Biolegend), anti-NKp44 (P44-8.1, BD), anti-NKp40 (29A1.4, Biolegend), anti-TNF-α (Mab11, eBioscience), anti-IFN-γ (485.B3, Biolegend). Annexin V/PI staining was performed according to the manufacturer’s protocol (Biolegend). The data was analyzed using FlowJo 10.7.1 software.
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