T 25flasks

Manufactured by Iwaki

Sourced in Japan

The T-25 flask is a laboratory cell culture vessel designed for the growth and maintenance of adherent cell lines. It has a surface area of 25 square centimeters and is made of transparent polystyrene material.

Automatically generated - may contain errors

Lab products found in correlation

0.2 μm syringe filter, by Merck Group (1 mentions) Streptomycin sulphate, by Thermo Fisher Scientific (1 mentions)

2 protocols using t 25flasks

Feederloss Culture of Human ESCs

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Mitotically inactivated buffalo FF cells (treated
with 10 μg/ml mitomycin-C) were cultured in T-25
flasks (Iwaki) with addition of ES medium for 7
days. Buffalo fibroblast-conditioned medium was
collected every day, centrifuged at 200 g for 3 minutes,
filtered with a 0.2 μm syringe filter (Millipore,
Watford, England) and frozen at -80˚C. After
thawing, the medium was equilibrated for 2 hours
at 5% CO₂ and 37˚C and then used for the feederfree
culture of human ES cells.

Zandi M., Muzaffar M., Shah S.M., Kumar Singh M., Palta P., Kumar Singla S., Manik R, & Chauhan M.S. (2015). Optimization of Buffalo (Bubalus bubalis) Embryonic Stem Cell Culture System. Cell Journal (Yakhteh), 17(2), 264-273.

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Cell Culture Protocols for PAEC, PMVEC, PSM and PF

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PAEC, PMVEC, PSM and PF cells were obtained from ScienCell (Germany) and grown in cell type-specific sterile growth medium (GM) in a humidified incubator at 37 °C and 5 % CO 2 in T25 flasks (Iwaki, Japan). In addition to supplied supplements, media were supplemented with: 200 μmol.l -1 glutamine dimer, 2 μg.ml -1 amphotericin-B, 60 μg.ml
penicillin-G and 100. μg ml -1 streptomycin sulphate (Invitrogen, UK). Medium was replaced aseptically every 3-4 days. Cells were sub-cultured every 10-14 days when ∼ 80-90 % confluent. Experiments were conducted at passage 4-8 at which point cells exhibited sufficient growth rates without loss of phenotype.

Holmes D., Corr M., Thomas G., Harbinson M., Campbell M., Spiers P, & Bell D. (2020). Protective effects of intermedin/adrenomedullin-2 in a cellular model of human pulmonary arterial hypertension. Peptides, 126.

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