Superdex 200 pc 3.2 column

Manufactured by GE Healthcare

The Superdex 200 PC 3.2 column is a size-exclusion chromatography column used for the separation and purification of proteins, peptides, and other biomolecules. It features a stationary phase that allows for the separation of molecules based on their size and molecular weight.

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Lab products found in correlation

Pageblue, by Thermo Fisher Scientific (1 mentions) Tris glycine sds page, by Bio-Rad (1 mentions) Superdex 75 pc3.2 column, by GE Healthcare (1 mentions)

Close spelling variants of this product

Superdex 200 (746 citations) Superdex 200 column (565 citations) Superdex 200 PC 3.2/30 column (22 citations) Superdex 200 Increase PC 3.2 column (2 citations) Superdex 200 PC 3.2/300 Increase column (2 citations)

2 protocols using superdex 200 pc 3.2 column

NisB-NisC-Prenisin Co-elution Analysis

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The co-elution studies were performed on a Äkta Micro system using a Superdex 200 PC 3.2 column (GE Healthcare) equilibrated with 50 mM HEPES-NaOH, pH 7.5, 500 mM NaCl with a flow rate of 0.05 mL/min.
A 50 μL reaction mixture consisting of 20 μM NisB, 160 μM NisC and 200 μM prenisin peptide variant was incubated for 1 h at 25 °C and subsequently applied to SEC analysis. Elution was observed at 280 nm. After co-elution, the corresponding fractions were analyzed by a 4–20% gradient Tris-Glycine SDS-PAGE (Biorad) gel stained with Page-Blue (Thermo Fisher).

Reiners J., Abts A., Clemens R., Smits S.H, & Schmitt L. (2017). Stoichiometry and structure of a lantibiotic maturation complex. Scientific Reports, 7, 42163.

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Size-Exclusion Chromatography of CI and RexA

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50 μl samples containing full-length CI protein at 125 μM, RexA protein at 125 μM, or a 1:1 mixture of both proteins was incubated for 15 minutes at room temperature and analyzed by SEC using a Superdex 200 PC 3.2 column (GE Healthcare) equilibrated in SEC buffer (20 mM HEPES, pH7.5, 150 mM KCl, 5 mM MgCl₂, and 1mM DTT). To assess CI and RexA interactions with DNA, samples containing the annealed double-stranded DNA substrates OR₁-OR₂ or OL₁-OL₂ were also prepared at 2:1.2 protein to DNA molar ratio. Individual DNA substrates were injected alone at a concentration of 75 μM for comparison. All eluted fractions were further analyzed by SDS-PAGE using 4 –20% gradient gels, and then silver-stained to visualize DNA and Coomassie-stained to visualize protein. Samples were similarly prepared for the D197G, NTD, and CTD CI constructs but were analyzed using a Superdex 75 PC 3.2 column (GE Healthcare) equilibrated in SEC buffer.

Thomason L.C., Schiltz C.J., Court C., Hosford C.J., Adams M.C., Chappie J.S, & Court D.L. (2021). Bacteriophage λ RexA and RexB Functions Assist the Transition from Lysogeny to Lytic Growth. Molecular microbiology, 116(4), 1044-1063.

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