To verify the protein levels of hub genes in human renal biopsy samples, we selected five DN cases and five minimal change disease (MCD) cases as controls. The current study was approved by the ethics committee of Beijing Friendship Hospital affiliated with Capital Medical University. All patients provided written informed consent. The formalin-fixed, paraffin-embedded renal sections were incubated with primary antibodies against ALOX5 (#3289, Cell Signal Technology), PTEN (ab267787, Abcam, Cambridge, UK), and NCF2 (ab109366, Abcam), then analyzed using the streptavidin peroxidase detection system (Abcam) according to the manufacturer’s protocol. We used DAB (Sinopharm, China) as the horseradish peroxidase (HRP)-specific substrate (chromogen).
Ab109366
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab109366 is a lab equipment product offered by Abcam. It is a tool designed for use in scientific research and laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab109366
1
Verification of Protein Levels in Renal Biopsy
2
Immunohistochemical Assessment of NCF2 in HCC
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Paraffin embedded HCC tissues of CHCAMS Cohort were used for immunohistochemistry (IHC). After deparaffinization and hydration, heat‐induced method was performed for antigen retrieval. Primary antibody of NCF2 (ab109366, 1:25, Abcam, USA) was incubated at 4°C overnight. Sections were washed with TBS‐T buffer, and then incubated with secondary antibody, and finally stained with DAB. Semiquantitative assessment of the stained sections were determined by the proportion of positive cells and the staining intensity: “−” = no positive cells; “+” = 0 ~ 25% and weak or moderate intensity; “++” = 25% ~ 50% and moderate intensity, or 0 ~ 25% and strong intensity, or 50% ~ 100% and weak intensity; “+++” = 50% ~ 100% and strong intensity. All the sections were scored independently by two experienced pathologists.
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