Anti actb

Manufactured by Beyotime

Sourced in Canada

Anti-ACTB is a laboratory reagent that specifically binds to and detects the ACTB protein, also known as beta-actin. ACTB is a widely expressed cytoskeletal protein that is often used as a reference or control in various biological experiments. Anti-ACTB can be used to quantify or visualize the ACTB protein in samples.

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Lab products found in correlation

Bca protein assay kit, by Beyotime (2 mentions) Enhanced chemiluminescence detection kit, by Beyotime (1 mentions) A0216, by Beyotime (1 mentions) A0208, by Beyotime (1 mentions) St506, by Beyotime (1 mentions) Anti p erk1 2, by ABclonal (1 mentions) Anti cyclin d1, by Cell Signaling Technology (1 mentions) Anti cdkn1a p21, by ABclonal (1 mentions) Anti erk1 2, by ABclonal (1 mentions) Sc 47739, by Santa Cruz Biotechnology (1 mentions) Anti puma, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions)

2 protocols using anti actb

Western Blot Analysis of Cell Signaling Proteins

Cited 1 time

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Cells were lysed with radioimmunoprecipitation assay lysis buffer with
phenylmethylsulfonyl fluoride (ST506, Beyotime Institute of Biotechnology).
Proteins were quantified using the BCA Protein Assay Kit (P0010, Beyotime
Institute of Biotechnology). Total protein (30 μg) was loaded onto
SDS–polyacrylamide gel electrophoresis gels and subsequently transferred
to poly(vinylidene fluoride) membranes (162-0177, Bio-Rad). After blocking with
5% nonfat milk in PBST (phosphate-buffered saline with Tween 20), membranes were
incubated with the following primary antibodies at the suggested dilutions:
anti-CDKN1A/p21 (10355-1-AP, ProteinTech Group), anti-CDKN2A/p16 (10883-1-AP,
ProteinTech Group), anti-ACTB (AA128, Beyotime Institute of Biotechnology), and
anti-ETS1 antibody (14069S, Cell Signaling Technology). Primary antibodies were
detected with horseradish peroxidase–labeled goat anti-rabbit (A0208,
Beyotime Institute of Biotechnology) or anti-mouse immunoglobulin G (IgG) (H +
L) (A0216, Beyotime Institute of Biotechnology) and developed with an Enhanced
Chemiluminescence Detection Kit (P0018, Beyotime Institute of Biotechnology)
(39 (link)).

Xiao F.H., Yu Q., Deng Z.L., Yang K., Ye Y., Ge M.X., Yan D., Wang H.T., Chen X.Q., Yang L.Q., Yang B.Y., Lin R., Zhang W., Yang X.L., Dong L., He Y., Zhou J., Cai W.W., Li J, & Kong Q.P. (2022). ETS1 acts as a regulator of human healthy aging via decreasing ribosomal activity. Science Advances, 8(17), eabf2017.

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Western Blot Analysis of Cell Signaling

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Cells were lysed with RIPA Lysis Buffer with PMSF (ST506, Beyotime Institute of Biotechnology). Proteins were quantified by BCA Protein Assay Kit (P0010, Beyotime Institute of Biotechnology). 20–80 μg of total protein was loaded onto SDS-PAGE and subsequently transferred to PVDF membranes (162-0177, Bio-Rad, Richmond, CA). After blocking with 5% nonfat milk or bovine serum albumin (BSA) in PBST, membranes were incubated with the following primary antibodies at the suggested dilutions: anti-CDKN1A/p21 (A1483, ABclonal, Cambridge, MA), anti-TP53/p53 (sc-6243, Santa Cruz Biotechnology, Santa Cruz, CA), anti-ERK1/2 (A0229, ABclonal), anti-p-ERK1/2 (AP0472, ABclonal), anti-p-Histone H2A.X (S139) (AP0099, ABclonal), anti-p-ATM (10H11.E12) (sc-47739, Santa Cruz Biotechnology), anti-p-CHK1 (Ser345) (sc-17922, Santa Cruz Biotechnology), anti-p-CHK2 (Thr68) (sc-16297-R, Santa Cruz Biotechnology), anti-PUMA (sc-374223, Santa Cruz Biotechnology), anti-TICRR (NBP2-41283, Novus Biologicals, Littleton, CO), anti-cyclin D1 (2922S, Cell Signaling Technology, Danver, MA) and anti-ACTB (AA128, Beyotime Institute of Biotechnology). Primary antibodies were detected with HRP-labeled goat anti-rabbit (A0208, Beyotime Institute of Biotechnology) or anti-mouse IgG (H+L) (A0216, Beyotime Institute of Biotechnology).

Yu Q., Pu S.Y., Wu H., Chen X.Q., Jiang J.J., Gu K.S., He Y.H, & Kong Q.P. (2019). TICRR Contributes to Tumorigenesis Through Accelerating DNA Replication in Cancers. Frontiers in Oncology, 9, 516.

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