Cellsens dimension software

Manufactured by Olympus

Sourced in Japan, Germany, United States, Canada, Italy

CellSens Dimension software is a comprehensive imaging and analysis platform developed by Olympus. It provides tools for capturing, processing, and analyzing microscopic images and data. The software offers a range of features to support various imaging applications, including live-cell imaging, time-lapse recording, and advanced image analysis.

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Lab products found in correlation

Bx43 microscope, by Olympus (14 mentions) Ix83 inverted microscope, by Olympus (13 mentions) Alexa fluor 488, by Thermo Fisher Scientific (8 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (8 mentions) Bx40 microscope, by Olympus (8 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (7 mentions) Ix83 microscope, by Olympus (7 mentions) Sybr gold, by Thermo Fisher Scientific (7 mentions) Dp72 microscope camera, by Olympus (7 mentions) Bx63 microscope, by Olympus (6 mentions) Ix83 fluorescent microscope, by Olympus (6 mentions) Triton x 100, by Merck Group (6 mentions) Bx61 microscope, by Olympus (6 mentions) Bx51 microscope, by Olympus (5 mentions) Orca flash 4.0 camera, by Hamamatsu Photonics (5 mentions) Ix73 microscope, by Olympus (4 mentions) Dp71 camera, by Olympus (4 mentions) Ix 73 inverted microscopy, by Olympus (4 mentions) Xm10 monochromatic ccd camera, by Olympus (4 mentions) Dp73 digital camera, by Olympus (3 mentions)

326 protocols using cellsens dimension software

Multimodal Microscopy Imaging Protocol

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Fluorescence and bright field microscope images were collected using a DP80 dual color/monochrome sensor CCD camera (Olympus America, Center Valley, PA) with CellSens Dimension software (Olympus Soft Imaging Solutions) with Extended Focal Imaging (EFI) function. Wide-filed images were also collected using defined scanning area mode with multiple image alignment (MIA) algorithm. Imaging experiments were repeated at least three times on independent sets of vector-injected mice. Confocal fluorescence microscope images were collected using an Olympus FV1000 confocal microscope equipped with an UPlanApo 100×/1.35 numerical aperture oil immersion objective and analyzed with Fluoview version 1.7a software (Olympus, Center Valley, PA). Collected images were processed into standard tagged image file (TIF) format using CellSens Dimension software (Olympus Soft Imaging Solutions) with Extended Focal Imaging (EFI) function.
Further Materials and Methods details are provided in the Supplementary Information.

Lu Z.H., Kaliberov S., Sohn R.E., Kaliberova L., Du Y., Prior J.L., Leib D.J., Chauchereau A., Sehn J.K., Curiel D.T, & Arbeit J.M. (2017). A new model of multi-visceral and bone metastatic prostate cancer with perivascular niche targeting by a novel endothelial specific adenoviral vector. Oncotarget, 8(7), 12272-12289.

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Retinal Vascular and Cellular Morphometry

Cited 3 times

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ADPase-stained retinal flatmounts were used to determine the tortuosity index and vessel diameter. For the tortuosity index, a line was traced along the tortuous arteries using the polyline tool compared to a straight line traced from the vessel origin at the optic disk to the branch point using the arbitrary line tool of the CellSens software (Olympus America Inc., Center Valley, PA, USA) as previously described [33 (link),34 (link),60 (link),61 (link)]. Vessel diameter at the optic disk was quantified using the arbitrary line tool of the CellSens Dimension software (Olympus America Inc., Center Valley, PA, USA). The number of endothelial cells present in the nerve fiber layer (NFL)/ganglion cell layer (GCL), the total retinal thickness, and the thickness of the NFL/GCL, inner plexiform layer (IPL), INL (inner nuclear layer), and ONL (outer nuclear layer) were quantified in the H&E stained sections using the count and measure of region of interest and arbitrary line tools of CellSens Dimension software (Olympus America Inc., Center Valley, PA, USA).

Guzel S., Cai C.L., Ahmad T., Quan M., Valencia G.B., Aranda J.V, & Beharry K.D. (2020). Bumetanide Suppression of Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy. International Journal of Molecular Sciences, 21(3), 987.

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Clonogenic Assay for Stem Cell and Cancer Cell Analysis

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Clonogenic assays were performed to analyze stem cell behavior and attachment-dependent colony formation and the growth of cancer cells as described previously [30 (link)]. Briefly, cells were seeded at low density (1000 cells/well in a 6-well plate) in triplicates, treated with HDACi at the indicated concentrations and incubated for 10 days at 37 °C. Subsequently, cells were rinsed 2 times with PBS and fixed for 30 min with 6% v/v glutaraldehyde and stained with 0.5% crystal violet simultaneously, washed with tap water and dried before microscopical analysis. The number and size of colonies were calculated with CellSens Dimension Software (Olympus Soft Imaging Solutions GmbH, Münster, Germany).

Freese K., Seitz T., Dietrich P., Lee S.M., Thasler W.E., Bosserhoff A, & Hellerbrand C. (2019). Histone Deacetylase Expressions in Hepatocellular Carcinoma and Functional Effects of Histone Deacetylase Inhibitors on Liver Cancer Cells In Vitro. Cancers, 11(10), 1587.

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Immunohistochemical Analysis of Ovarian Samples

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The ovarian samples of mice were fixed, paraffin embedded, and processed in 5-μm-thick serial sections. After deparaffinization and rehydration through ethanol with gradient concentration (100–75%), the sections were treated with 3% H₂O₂ at room temperature and microwaved for 15 min in citrate buffer for antigen retrieval. Sections were incubated with primary anti-rabbit Lcp2 (1:200, Proteintech, China), anti-rabbit H2-Ab1 (1:200, Abclonal, China) antibody at 4°C overnight, and secondary horseradish peroxidase-labeled goat anti-rabbit IgG (SA1022, Boster, Wuhan, China) for 30 min at room temperature. With DAB chromogenic agent (AR1022, Boster, Wuhan, China) and subsequent counterstaining by hematoxylin, the sections were visualized. The images were captured by using the cellSens Dimension software (Olympus Soft Imaging Solutions GmbH; Germany), and the relative expression was analyzed by Image Pro Plus 6.0 software (Media Cybernetics, Silver Spring, MD, United States).

Ma L., Lu H., Chen R., Wu M., Jin Y., Zhang J, & Wang S. (2020). Identification of Key Genes and Potential New Biomarkers for Ovarian Aging: A Study Based on RNA-Sequencing Data. Frontiers in Genetics, 11, 590660.

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Quantifying Muscle Fiber Cross-Sections

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Muscle cross-sections (10 µm thick) were obtained using a cryostat (HM500M Microm International, Fisher Scientific, Illkirch, France) at −20 °C. Cross-sections were labeled with anti-laminin-α1 (L9393 Sigma, Saint-Quentin-Fallavier, France) to outline the fibers, and resolved with corresponding secondary antibodies conjugated to Alexa-Fluor 488 (Invitrogen, Cergy-Pontoise, France). Observations and image acquisitions were captured with a high-resolution ORCA-Flash4.0 LT+ Digital CMOS camera coupled to an IX-73 microscope (Olympus) and Cell-Sens dimension software (Olympus Soft Imaging Solutions, Münster, Germany). The cross-sectional area (CSA) was determined for each fiber, using ImageJ 1.53f51 (http://rsb.info.nih.gov/ij/ accessed on 17 May 2022).

Carraro V., Combaret L., Coudy-Gandilhon C., Parry L., Averous J., Maurin A.C., Jousse C., Voyard G., Fafournoux P., Papet I, & Bruhat A. (2022). Activation of the eIF2α-ATF4 Pathway by Chronic Paracetamol Treatment Is Prevented by Dietary Supplementation with Cysteine. International Journal of Molecular Sciences, 23(13), 7196.

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Investigating Fungal Growth and Stress Responses

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Colony growth and microsclerotia formation on media containing 2% agar were studied after point-inoculation of 5 × 10⁴ spores and incubation at 25 °C for ten days. PDM, SXM, and Czapek-Dox medium (CDM, modified from [44 (link)] as described by [45 (link)]) were used. Morphology under different stress conditions was tested by replacing sucrose in CDM with 3% cellulose or supplementing CDM with 0.8 M sorbitol, 0.5 M sodium chloride, 0.004% SDS, or 0.000075% hydrogen peroxide. Ex-planta phenotypes were examined by binocular microscopy (SZX12-ILLB2-200, illuminated with KL1500-LCD light source and equipped with SC30 camera, Olympus, Tokyo, Japan) with cellSens Dimension software (Olympus, Tokyo, Japan).

Maurus I., Leonard M., Nagel A., Starke J., Kronstad J.W., Harting R, & Braus G.H. (2022). Tomato Xylem Sap Hydrophobins Vdh4 and Vdh5 Are Important for Late Stages of Verticillium dahliae Plant Infection. Journal of Fungi, 8(12), 1252.

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Cellular Uptake of DOX Nanoparticles

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J774A.1
cells were seeded with a density of 5 × 10⁵ cells
per well on poly-d-lysine-precoated glass coverslips, placed
inside wells of a 6-well tissue culture plate, and incubated overnight.
On the second day, cell culture medium was replaced with 2.5 mL of
50 μM DOX in various nanoparticles (i.e., DOX-NPs, DOX-M-NPs,
DOX-AI-M-NPs, and DOX-AS-M-NPs). Cells were incubated for 20 min,
followed by five additional minutes of incubation with 50 μM
Hoechst 33342 in a 37 °C incubator and protected from light.
Cells were then washed with PBS three times, fixed with 4% paraformaldehyde,
and observed under a fluorescence microscope (Olympus BX 53, Center
Valley, PA) connected to the Olympus cellSens Dimension software.
To investigate the effect of pH on the cellular uptake, nanoparticles
were preincubated with PBS (pH 6.8) at 37 °C for 6 h before further
incubating with cells.
In order to track the intracellular fate
of nanoparticles, DOX-AS-M-NPs were prepared with 5% (w/w) of PLGA
752H that was conjugated with fluorescein isothiocyanate (FITC).³⁴ Cells were incubated with nanoparticles for
15, 30, or 60 min, followed by five additional minutes of incubation
with Hoechst 33342. Cells were then washed, fixed, and observed under
a microscope as mentioned above.

Niu M., Naguib Y.W., Aldayel A.M., Shi Y.C., Hursting S.D., Hersh M.A, & Cui Z. (2014). Biodistribution and in Vivo Activities of Tumor-Associated Macrophage-Targeting Nanoparticles Incorporated with Doxorubicin. Molecular Pharmaceutics, 11(12), 4425-4436.

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Evaluation of Myelinated Nerve Fibers

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The dissected sciatic nerves were fixed with 2.5% glutaraldehyde overnight at 4 °C and postfixed with 1% osmium tetroxide (OsO4) for 2 h, dehydrated, and embedded in epoxy resin. Semi-thin sections (1 µm) were cut vertically with an ultramicrotome (EM UC7i, Leica Microsystems, Denver, CO, http://www.leica-microsystems.com) and stained with 1% toluidine blue solution, and images were captured under a light microscope (Olympus IX-73). The density of the myelinated fibers (fibers/1000 µm²) was analyzed from six non-overlapping visual fields per specimen. On the other hand, ultrathin sections (60 nm) were stained with lead citrate and uranyl acetate, and images were captured under a transmission electron microscope (TEM, JEM-1400). All these services were provided by the Electron Microscopy Resource Lab of Perelman School of Medicine at UPenn. The diameter of myelinated fibers, axons, and the thickness of the myelin sheath was evaluated by cellSens Dimension software (Olympus), and the G-ratio was calculated as the ratio of the inner axonal diameter to the total outer diameter of the fiber.

Zhang Q., Burrell J.C., Zeng J., Motiwala F.I., Shi S., Cullen D.K, & Le A.D. (2022). Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves. Stem Cell Research & Therapy, 13, 263.

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Immunostaining Identification of Neural Cells

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Coronal and sagittal sections were deparaffinized and immunostained using anti-tyrosine hydroxylase (TH; 1:500; Millipore AB152) to identify dopaminergic neurons, anti-glial fibrillary acidic protein (GFAP; 1:500; DAKO Z0334) to identify astrocytes and anti-calcium adaptor binding protein 1 (Iba-1; 1:250; WAKO 016-20001) to label microglia, per our previously published methods (Hammond et al., 2017 (link); Miller et al., 2011 (link)). Sections were visualized by automated montage imaging of individual 10X frames of each immunostained section using a Hammatsu Flash4.0 digital CMOS camera, ProScan III stage controller (Prior, Rockland, MA USA) and CellSens Dimension software (version 1.12, Olympus, Center Valley, PA, USA). Regions of interest representing distinct anatomical nuclei were analyzed on each composite montage image.

Bantle C.M., Rocha S.M., French C.T., Phillips A.T., Tran K., Olson K.E., Bass T.A., Aboellail T., Smeyne R.J, & Tjalkens R.B. (2021). Astrocyte inflammatory signaling mediates α-synuclein aggregation and dopaminergic neuronal loss following viral encephalitis. Experimental neurology, 346, 113845.

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Yeast Cell Budding and Viability Analysis

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For verification of the cells budding, 20 µL of the cell suspensions were spotted on the plate with solid YPD or YPGal medium, and the pictures of the cells were taken using the Nikon Eclipse E200 microscope equipped with the Olympus DP26 digital camera at the beginning of the experiment and after 0 h, 24 h, and 48 h.
For determining death cells, staining with PI was used. Cells were suspended in PBS and stained with 5 μg/mL propidiumiodide (Sigma-Aldrich, Saint-Louis, MO, USA) for 15 min in the dark at room temperature. Fluorescence pictures were taken with Olympus BX-51 microscope equipped with a DP-72 digital camera and cellSens Dimension software (Olympus, Tokyo, Japan). Dead cells were red fluorescent.

Borkiewicz L., Mołoń M., Molestak E., Grela P., Horbowicz-Drożdżal P., Wawiórka L, & Tchórzewski M. (2019). Functional Analysis of the Ribosomal uL6 Protein of Saccharomyces cerevisiae. Cells, 8(7), 718.

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