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Envision polymer hrp anti mouse 3 3 diaminobenzidine dab

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The Envision Polymer-HRP anti-mouse/3,3′diaminobenzidine (DAB) is a laboratory equipment product from Agilent Technologies. It is designed for immunohistochemical detection of mouse primary antibodies in tissue sections.

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Lab products found in correlation

Er 6f11, by Leica (2 mentions)

3 protocols using envision polymer hrp anti mouse 3 3 diaminobenzidine dab

1

Immunohistochemistry Protocol for Tissue Analysis

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For immunohistochemistry (IHC) cut tissue sections (5μm) on charged glass slides were baked for 10–12 hours at 58°C in a dry slide incubator, deparaffinized in xylene and rehydrated via an ethanol step gradient. Heat-induced antigen retrieval steps were performed at pH 9.0 for all targets. All primary antibodies were incubated at room temperature for 1 hour [clone, manufacturer, dilution: Her2 (SP3, Neomarkers, 1:100); ER (6F11, Leica, 1:200); CD3 (polyclonal, Dako, 1:100)] followed by a standard chromogenic staining protocol with the Envision Polymer-HRP anti-mouse/3,3′diaminobenzidine (DAB, Dako) process. Slides were counterstained in Harris hematoxylin. Immunohistochemistry scoring was performed using established guidelines, when appropriate. All IHC results were evaluated against positive and negative tissue controls.
Krug K., Jaehnig E.J., Satpathy S., Blumenberg L., Karpova A., Anurag M., Miles G., Mertins P., Geffen Y., Tang L.C., Heiman D.I., Cao S., Maruvka Y.E., Lei J.T., Huang C., Kothadia R.B., Colaprico A., Birger C., Wang J., Dou Y., Wen B., Shi Z., Liao Y., Wiznerowicz M., Wyczalkowski M.A., Chen X.S., Kennedy J.J., Paulovich A.G., Thiagarajan M., Kinsinger C.R., Hiltke T., Boja E.S., Mesri M., Robles A.I., Rodriguez H., Westbrook T.F., Ding L., Getz G., Clauser K.R., Fenyö D., Ruggles K.V., Zhang B., Mani D.R., Carr S.A., Ellis M.J, & Gillette M.A. (2020). Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy. Cell, 183(5), 1436-1456.e31.
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2

Immunohistochemistry Staining Protocol

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Tissue sections on charged glass slides were cut to 5 µm and deparaffinised in xylene and rehydrated via an ethanol step gradient. Peroxidase blocking, heat-induced antigen retrieval, and primary antibody incubation were performed per standard protocol under the following abbreviated conditions: ERBB2 (SP3, Neomarkers) 1:100, Tris pH 9.0; AR (441, sc-7305, Santa Cruz) 1:50, Tris pH 9.0; Muc1 (sc-7313, Santa Cruz) 1:150, Citrate pH 6.0; CD3 (polyclonal, A0452, Dako) 1:100, Tris pH 9.0. All primary antibodies were incubated at room temperature for 1 h followed by standard chromogenic staining with the Envision Polymer-HRP anti-mouse/3,3′diaminobenzidine (DAB; Dako) process. Immunohistochemistry scoring were performed using established guidelines, when appropriate. All IHC results were evaluated against positive and negative controls.
Satpathy S., Jaehnig E.J., Krug K., Kim B.J., Saltzman A.B., Chan D.W., Holloway K.R., Anurag M., Huang C., Singh P., Gao A., Namai N., Dou Y., Wen B., Vasaikar S.V., Mutch D., Watson M.A., Ma C., Ademuyiwa F.O., Rimawi M.F., Schiff R., Hoog J., Jacobs S., Malovannaya A., Hyslop T., Clauser K.R., Mani D.R., Perou C.M., Miles G., Zhang B., Gillette M.A., Carr S.A, & Ellis M.J. (2020). Microscaled proteogenomic methods for precision oncology. Nature Communications, 11, 532.
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3

Immunohistochemistry Protocol for Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemistry (IHC) cut tissue sections (5μm) on charged glass slides were baked for 10–12 hours at 58°C in a dry slide incubator, deparaffinized in xylene and rehydrated via an ethanol step gradient. Heat-induced antigen retrieval steps were performed at pH 9.0 for all targets. All primary antibodies were incubated at room temperature for 1 hour [clone, manufacturer, dilution: Her2 (SP3, Neomarkers, 1:100); ER (6F11, Leica, 1:200); CD3 (polyclonal, Dako, 1:100)] followed by a standard chromogenic staining protocol with the Envision Polymer-HRP anti-mouse/3,3′diaminobenzidine (DAB, Dako) process. Slides were counterstained in Harris hematoxylin. Immunohistochemistry scoring was performed using established guidelines, when appropriate. All IHC results were evaluated against positive and negative tissue controls.
Krug K., Jaehnig E.J., Satpathy S., Blumenberg L., Karpova A., Anurag M., Miles G., Mertins P., Geffen Y., Tang L.C., Heiman D.I., Cao S., Maruvka Y.E., Lei J.T., Huang C., Kothadia R.B., Colaprico A., Birger C., Wang J., Dou Y., Wen B., Shi Z., Liao Y., Wiznerowicz M., Wyczalkowski M.A., Chen X.S., Kennedy J.J., Paulovich A.G., Thiagarajan M., Kinsinger C.R., Hiltke T., Boja E.S., Mesri M., Robles A.I., Rodriguez H., Westbrook T.F., Ding L., Getz G., Clauser K.R., Fenyö D., Ruggles K.V., Zhang B., Mani D.R., Carr S.A., Ellis M.J, & Gillette M.A. (2020). Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy. Cell, 183(5), 1436-1456.e31.
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