Total RNA was isolated from all samples (approximately 100 mg) using the RiboEx Total RNA reagent (GeneAll Biotechnology, South Korea). The amount of extracted RNA was quantified by measuring the absorbance at 260 nm. The purity of the RNA was determined by calculating the ratio of the absorbance at 260 and 280 nm. The absence of degradation of the RNA was confirmed by electrophoresis of the RNA on a 1% agarose gel containing ethidium bromide.
Riboex total rna reagent
Manufactured by GeneAll
Sourced in Cameroon
RiboEx Total RNA reagent is a reagent for the isolation of total RNA from various biological samples. It is a monophasic solution of phenol and guanidinium isothiocyanate that provides effective lysis and denaturation of cells and tissues for efficient RNA extraction.
Automatically generated - may contain errors
2 protocols using riboex total rna reagent
1
Total RNA Isolation and Characterization
2
RNA Isolation and qPCR Analysis
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RiboEx Total RNA reagent (Geneall biotechnology, Seoul, Korea) was used for RNA isolation from liver, muscle, and adipose tissue. cDNA was synthesized using Moloney Murine Leukemia Virus (MMLV) reverse transcriptase kit (Bioneer, Daejeon, Korea). The Rotor-Gene 3000 (Corbett Research, Sydney, Australia) was utilized to conduct quantitative polymerase chain reaction (qPCR) by using the AccuPower 2X Greenstar qPCR Master Mix (Bioneer, Daejeon, Korea). Gene expression was normalized using β-actin, a housekeeping gene and expressed as fold change of the HF group followed by the 2 − ∆∆Ct method [29 (link)]. Primer sequences used for this study are listed in Table S2 .
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