Blood was collected from the inferior vena cava at euthanasia. BUN was measured on the Beckman Coulter AU 680 analyzer. Blood counts were performed on the IDEXX Procyte DX analyzer. Both procedures were performed with the assistance of the Weill Cornell Medicine Laboratory of Comparative Pathology. Serum iron levels were measured using SFBC kits (Biolabo) following manufacturer’s instructions. We used ELISA to measure serum cystatin C (R&D Systems, Bio-Techne), serum soluble TfR (MyBioSource), and urine albumin (Abcam) following manufacturers’ instructions.
Serum cystatin c
Manufactured by R&D Systems
Sourced in United States
Serum cystatin C is a lab equipment product that measures the concentration of cystatin C, a small protein, in the blood. Cystatin C is a marker of kidney function and can be used to assess the glomerular filtration rate, which is the rate at which the kidneys filter waste from the blood.
Automatically generated - may contain errors
3 protocols using serum cystatin c
1
Comprehensive Blood and Urine Analysis
2
Biomarkers of Cardiac Surgery Injury
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Serum and urine samples were obtained preoperatively, hour 6 after CPB, and daily on PODs 1 to 4. Serum samples were centrifuged at 1227g for 15 minutes at 4 °C. Urine samples were centrifuged at 626g for 30 minutes at 4 °C. Serum and urine aliquots were stored at −80 °C until thawed for batch analysis. Urine creatinine concentrations were measured by capillary electrophoresis. Enzyme-linked immunosorbent assays were used to measure serum cystatin C (R&D Systems, Minneapolis, MN), urine KIM-1 (R&D Systems), and urine NGAL (BioPorto Diagnostics, Hellerup, Denmark). The lower limit of detection for urine KIM-1 was 0.156 ng/ml. Of the total of 598 urine samples, only 6 values were below this limit, and these values were analyzed as being 0.156 ng/ml. Measurements of cystatin C and NGAL were not below the lower limits of detection for their respective assays. Urine NGAL and KIM-1 values were normalized by urine Cr level.
3
Serum Biomarkers of Oxidative Stress
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Enzyme-linked immunosorbent assay (ELISA) kits were used to estimate serum cystatin C (R&D Systems, USA), neutrophil gelatinase-associated lipocalin (NGAL), NADPH oxidase, nuclear factor erythroid 2-related factor 2 (Nrf2) (Cusabio, China), hemeoxygenase-1(HO-1) (Enzo Life Sciences, Egypt), heat shock protein 90 (HSP 90) (Bioassay™, USA), nuclear factor kappa-B (NF-κB) (Eiaab, China) and tumor necrosis factor-α (TNF-α) (Raybio, USA). Reduced glutathione (GSH) and blood urea nitrogen (BUN) were estimated using glutathione reduced and blood urea nitrogen colorimetric kits, respectively (Biodiagnostic, Egypt). Total antioxidant capacity (TAC) was assessed by antioxidant assay kit (Zenbio, USA).
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