For the UPLC-QToF-MS analysis, the powdered red ginseng samples were extracted in a manner similar to our previous studies using ultrasonic extraction [13 (link)]. In total, 300 mg of each powered red ginseng was weighed in a centrifuge tube (15 mL, PP-single use; BioLogix Group, Jinan, Shandong, China) and shaken vigorously after the addition of 6 mL of methanol. The extract was then placed in an ultrasonic cleaner (60 Hz; Wiseclean, Seoul, Korea) for 30 min. The solution was centrifuged (Legand Mach 1.6R; Thermo, Frankfurt, Germany) at a speed of 3000 rpm for 10 min, and an aliquot of the supernatant solution was filtered (0.2 µm; Acrodisk, Gelman Sciences, Ann Arbor, MI, USA) and injected into the UPLC system (Waters Co., Milford, MA, USA).
Legand mach 1.6r
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Legand Mach 1.6R is a high-performance bench-top centrifuge designed for a wide range of applications in laboratories. It features a maximum speed of 16,000 rpm and a maximum RCF of 28,000 x g. The centrifuge is equipped with a brushless induction motor and an advanced microprocessor control system for precise speed and time regulation.
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3 protocols using legand mach 1.6r
1
Red Ginseng UPLC-QToF-MS Analysis
2
Analysis of Rehmanniae Radix Bioactive Compounds
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After weighing approximately 0.1g of uniformed Rehmanniae Radix powder, it was melted into 40mL of 30% Methanol, and the mixture was centrifuged after ultrasonic extraction. After passing it through Syringe filter (0.2 μm), it was used as the test solution and was analyzed with HPLC/UV Detector. The HPLC was Alliance 2695 HPLC system, Waters, USA; the ultrasonic cleaner was SD-200H (Seong Dong Ultrasonics, Korea); the centrifugal separator was Legand Mach 1.6R (Thermo, Germany); 50ml tubes were used; the agitation was done at 4000r/min. For the preparation of the standard solutions, adequate amounts of Catalpol(Sigma, Cat.No. 50839) and 5-HMF(Sigma,Cat.No. H40807) were diluted with Methanol and used [16 (link)].
3
Ginseng Powder Extraction Protocol
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Sugar, amino-sugar, and maltol were extracted as follows. A 100 mg sample of ginseng powder was weighed in a centrifugal tube (15 mL, polypropylene-single use; BioLogix Group) and shaken vigorously after the addition of 10 mL of deionized water. Extraction was performed in an ultrasonic cleaner (60 Hz, Wiseclean) for 30 min. After ultrasonic extraction, centrifugal separation (Legand Mach 1.6R; Thermo) was performed for 10 min at 3000 rpm. The resulting supernatant solution was filtered (0.2 μm, Acrodisk; Gelman Sciences) and this filtrate was used as analytical solution for sugar, amino-sugar, amino acid, and maltol.
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