Acetylthiocholine iodide achi

Manufactured by Merck Group

Sourced in United States

Acetylthiocholine iodide (AChI) is a chemical compound used in laboratory settings. It is a substrate for the enzyme acetylcholinesterase, which is responsible for the breakdown of the neurotransmitter acetylcholine. AChI can be used to measure the activity of acetylcholinesterase in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (2 mentions) Sodium acetate, by Merck Group (1 mentions) Potassium persulphate, by Merck Group (1 mentions)

Spelling variants of this product

Acetylthiocholine iodide (310 citations) Acetylthiocholine (44 citations)

3 protocols using acetylthiocholine iodide achi

Synthesis and Evaluation of DBL

Check if the same lab product or an alternative is used in the 5 most similar protocols

The synthesis of DBL was carried out at the Niigata University of Pharmacy and Applied Life Sciences, as described previously [7 (link)]. The yellow DBL was evaluated by HPLC and the purity was 99.9%. Acetylthiocholine iodide (AChI) and 5,5’-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals used in the study were of analytical grade. Solutions of the drug and chemicals were freshly prepared before use.

Giridharan V.V., Karupppagounder V., Arumugam S., Nakamura Y., Guha A., Barichello T., Quevedo J., Watanabe K., Konishi T, & Thandavarayan R.A. (2020). 3,4-Dihydroxybenzalacetone (DBL) Prevents Aging-Induced Myocardial Changes in Senescence-Accelerated Mouse-Prone 8 (SAMP8) Mice. Cells, 9(3), 597.

+ Open protocol

+ Expand

Acetylcholinesterase Activity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acetylcholinesterase (AChE) type VI-S from Electrophorus electricus (electric eel), EC 3.1.1.7, 245 U/mg solid, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) and acetylthiocholine iodide (AChI) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Ascorbic acid was purchased from Fluka, Buchs, Switzerland.

Elshafie H.S., Caputo L., De Martino L., Sakr S.H., De Feo V, & Camele I. (2021). Study of Bio-Pharmaceutical and Antimicrobial Properties of Pomegranate (Punica granatum L.) Leathery Exocarp Extract. Plants, 10(1), 153.

+ Open protocol

+ Expand

Antioxidant and Acetylcholinesterase Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

2,2'-Diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3ethylbenzothiazine-6-sulfonic acid) (ABTS), 6-hydroxy2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tripyridyl-s-triazine (TPTZ), Fe-Cl 3 •6H 2 O, sodium acetate, potassium persulphate, acetylcholinesterase (AChE) type VI-S, from electric eel 349 U/mg solid, 411 U/mg protein, acetylthiocholine iodide (AChI) were purchased from Sigma-Aldrich. All the others chemicals including the solvents were of analytical grade.

Nassar-Eddin G., Zheleva‐Dimitrova D., Danchev N., & Simeonova R. (2021). Antioxidant and enzyme-inhibiting activity of lyophilized extract from Clinopodium vulgare L. (Lamiaceae). Pharmacia, 68(1), 259-263.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!