The hermaphrodite gland tissues of ten snails were employed. Testosterone and estradiol hormone levels were measured for all groups according to manufacturers instructions of the testosterone EIA kit (Enzo Life Science, Michigan, USA, ADI-900-065) and the estradiol EIA kit (Cayman Chemical Company, Michigan, USA, item no. 582251). Briefly, using fresh, disposable tips, 25 µl of each standard, control, and treated samples were dispensed into the proper wells of a microplate coated with either T or E monoclonal antibody. The 200 µl enzyme conjugate was then added to each microplate well, carefully mixed, and allowed to sit at room temperature for 60 minutes. The microplates were then processed, and absorbance was measured (Gholib et al., 2019 (link)).
Testosterone eia kit
Manufactured by Enzo Life Sciences
Sourced in United States
The Testosterone EIA kit is a laboratory equipment product that is used to measure the concentration of testosterone in a sample. It is a quantitative enzyme immunoassay (EIA) test designed to provide accurate and reliable results for research purposes.
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Lab products found in correlation
2 protocols using testosterone eia kit
1
Quantification of Snail Hormone Levels
2
Snail Hermaphrodite Gland Hormone Assay
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Tissues of the hermaphrodite glands of ten snails were used in this experiment. Testosterone (T) and estradiol hormones (E) concentrations were measured for all groups according to the manufacturer's recommendations, for testosterone EIA kit (Enzo Life Science, Michigan, USA, ADI-900-065) and for estradiol EIA kit (Cayman Chemical Company, Michigan, USA, item no. 582251). 25 μl of each standard, control, and samples (serum) in duplicate were dispensed with new disposable tips into appropriate wells on a microplate coated with T or E monoclonal antibody. After that, each microplate well was filled with 200 μl enzyme conjugate, thoroughly mixed, and then incubated for 60 min at room temperature. Following this, the microplates were treated and absorbance was measured (Gholib et al., 2020 (link)).
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