Dulbecco s modified eagle s medium dmem with glumax

The DPPH^●, ABTS^●+, 1,10-phenanthroline, H₂O₂, dimethyl sulfoxide (DMSO), 95% ethanol, ascorbic acid, trypan blue 0.4%, and lipopolysaccharide (LPS) were purchased from Sigma (Rowville, Australia) and Lomb Scientific Pty Ltd. (Sydney, Australia). The foetal bovine serum (FBS), antibiotics, and Dulbecco’s modified Eagle’s medium (DMEM) with gluMax were purchased from BD Bioscience (USA). The tumor necrosis factor-α (TNF-α) and interleukin (IL-6) (mouse)—ELISA standards and antibodies were also purchased from BD Bioscience.

The DPPH^●, ABTS^●+, 1,10-phenanthroline, H₂O₂, dimethyl sulfoxide (DMSO), 95% ethanol, ascorbic acid, 0.4% trypan blue, and lipopolysaccharide (LPS) were purchased from Sigma (Australia) and Lomb Scientific Pty Ltd. (Australia). The foetal bovine serum (FBS, Gibco, Australia), CCK-8 kits (Beyotime Institute of Biotechnology, China), antibiotics, and Dulbecco's modified Eagle's medium (DMEM) with gluMax were purchased from BD Bioscience. The other reagentswere tris-HCl (AMRESCO, USA), 2-hydroxy-1-ethanethiol (Sigma Aldrich, USA), sodium dodecyl sulfate (SDS, AMRESCO, USA), anti-IL-6 (CST, USA), anti-TNF-α (ABCAM, USA), anti-GAPDH (CST, USA), and albumin bovine V (AMRESCO, USA). The TNF-α and IL-6 (mouse), ELISA standards and antibodies were purchased from BD Bioscience (USA).