Human pd l1 antibody

Manufactured by Cell Signaling Technology

The Human PD-L1 Antibody is a laboratory reagent used for the detection and analysis of the PD-L1 protein in human samples. PD-L1 is an important immune checkpoint molecule that plays a role in regulating the immune response. The antibody can be used in various applications, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and distribution of PD-L1 in different cell types and tissues.

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Lab products found in correlation

Pbrm1 antibody, by Cell Signaling Technology (1 mentions) Jak1 antibody, by Cell Signaling Technology (1 mentions) Jak2 antibody, by Cell Signaling Technology (1 mentions) Phospho jak2 antibody, by Cell Signaling Technology (1 mentions) Stat1 antibody, by Cell Signaling Technology (1 mentions) β actin antibody a1978, by Merck Group (1 mentions) Phospho stat1 antibody, by Cell Signaling Technology (1 mentions) Human ifnγ 285 if 100, by R&D Systems (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions) Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Volume one software, by Bio-Rad (1 mentions) Goat anti mouse igg antibody, by Bio-Rad (1 mentions) Pvdf filter, by Bio-Rad (1 mentions) Mem per plus membrane protein extraction kit, by Thermo Fisher Scientific (1 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) Versadoc imaging system, by Bio-Rad (1 mentions)

Spelling variants of this product

PD-L1 (184 citations) PD-L1 antibody (11 citations) Primary anti-human PD-L1 antibody (4 citations) Rabbit anti‐human PD‐L1 antibody (2 citations)

2 protocols using human pd l1 antibody

Comprehensive Immune Marker Antibody Panel

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PBRM1 antibody (A301-591A) was from Bethyl Laboratories. Phospho-STAT1 antibody (clone ST1P-11A5; Tyr701; 33-3400), human CD3 antibody (clone F7.2.38; MA5-12577), human CD45RO antibody (clone UCHL1; MA5-11532), human CD4 antibody (clone 4B12; MS1528S0), and human CD8 antibody (clone C8/144B; MS457S0) were from ThermoFisher Scientific. Mouse CD3 antibody (D4V8L; 99940), mouse CD8 antibody(D4W22; 98941), mouse CD4 antibody (D7D2Z; 25229), human PD-L1 antibody (E1L3N; 13684), mouse PD-L1 antibody (D5V3B; 64988), mouse PD-1 antibody (D7D5W; 84651), PBRM1 antibody (D3F7O, 91894), JAK1 antibody (6G4, 3344), JAK2 antibody (D2E12, 3230), Phospho-JAK1 antibody (D7N4Z, Tyr1034/1035, 74129), Phospho-JAK2 antibody (C80C3, Tyr1007/1008, 3776), STAT1 antibody (D1K9Y, 14994), Phospho-STAT1 antibody (58D6, Tyr701, 9167), Phospho-STAT1 antibody (D3B7, Ser727, 8826), IRF1 antibody (D5E4, 8478), and BRG1 antibody (E9O6E; 52251) were from Cell Signaling Technology. IFNGR1 antibody (112802) was from BioLegend. IFNGR2 antibody (Cat No. GTX 64548) was from GeneTex. β-actin antibody (A1978) was from Sigma. Human IFNγ (285-IF-100) and mouse IFNγ (8234-MB-010) were from R&D Systems.

Liu X.D., Kong W., Peterson C.B., McGrail D.J., Hoang A., Zhang X., Lam T., Pilie P.G., Zhu H., Beckermann K.E., Haake S.M., Isgandrova S., Martinez-Moczygemba M., Sahni N., Tannir N.M., Lin S.Y., Rathmell W.K, & Jonasch E. (2020). PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma. Nature Communications, 11, 2135.

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Membrane Protein Extraction and Western Blot Analysis

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Membrane proteins were isolated with Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific), and cytoplasmic proteins were extracted with NE-PER™ Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific). We quantified the proteins with the DC Protein Assay kit (Bio-Rad, Hercules CA, USA). Denatured proteins were separated on a 10% SDS-PAGE gel and then transferred to PVDF filters (Bio-Rad). After blocking with 5% non-fat milk-TBST (Bio-Rad), the blots were incubated mouse PD-L1 antibody (Abcam), human PD-L1 antibody (Cell signaling), β-actin antibody (Cell signaling), CD63 polyclonal antibody (Abcam), or Calnexin polyclonal antibody (Abcam) at 4°C overnight, respectively. After washing 3 times with 1×TBST (Bio-Rad), the blots were incubated with peroxidase-linked secondary goat anti-rabbit IgG antibody (Bio-Rad), or goat-anti-mouse IgG antibody (Bio-Rad) were incubated for 1 h at room temperature. Finally, after incubation with SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific, Worcester, MA, USA), visual imaging was performed by VersaDoc™ Imaging System (Bio-Rad). Volume One software (Bio-Rad) was used for the relative quantification of blotted proteins.

Yi B., Cheng H., Wyczechowska D., Yu Q., Li L., Ochoa A.C., Riker A.I, & Xi Y. (2021). Sulindac modulates the response of proficient MMR colorectal cancer to anti-PD-L1 immunotherapy. Molecular cancer therapeutics, 20(7), 1295-1304.

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