Infinite f200 pro microplate reader

Manufactured by Tecan

Sourced in Switzerland, Austria, Germany

The Infinite F200 PRO is a high-performance microplate reader designed for a wide range of absorbance, fluorescence, and luminescence applications. It features a xenon flash lamp, a temperature-controlled incubation chamber, and automated plate handling capabilities.

Automatically generated - may contain errors

Lab products found in correlation

58 protocols using infinite f200 pro microplate reader

Sialidase Activity Measurement Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the determination of sialidase activity bound to avidin beads, beads were washed with PBS and directly resuspended in a reaction buffer containing 20 mM MES (pH 4.5) and 200 µM 2-O-(p-nitrophenyl)-α-d-N-acetylneuraminic acid for 4 h at 37 °C in the dark. Reactions were stopped by adding 1 M Na₂CO₃ and the absorbance measured using an Infinite F200 PRO microplate reader (TECAN).
Sialidase activity at the plasma membrane of macrophages transfected, or not, with negative control siRNA or NEU1 siRNA, was performed as described previously for human fibroblasts [7 (link)]. Cells, seeded in 12-well culture dishes (5 × 10⁵ cells/well), were washed with PBS and incubated with a reaction buffer containing 20 mm CH₃COONa (pH 6.5) and 400 µM 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid, with or without kE (50 µg/mL), for 2 h at 37 °C in the dark. After incubation, the reaction was stopped by adding 0.4 M glycine buffer (pH 10.4), and the fluorescent 4-methylumbelliferone product released in the medium was measured using the Infinite F200 PRO microplate reader (TECAN).

Kawecki C., Bocquet O., Schmelzer C.E., Heinz A., Ihling C., Wahart A., Romier B., Bennasroune A., Blaise S., Terryn C., Linton K.J., Martiny L., Duca L, & Maurice P. (2018). Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex. Cellular and Molecular Life Sciences, 76(4), 791-807.

+ Open protocol

+ Expand

Neuroprotective Effects of VPA Against Oxidative Stress

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

This experiment assessed the neuroprotective effect of VPA under glutamate and hydrogen peroxide (H₂O₂)-induced neurotoxic effects using the NSC-34 cell lines. After 70–80% confluence, the NSC-34 cell lines were exposed to H₂O₂ (300 µM) and glutamate (2 mM) with or without VPA (2 mM) addition, and were then incubated for 24 h. Then, an MTT [3‐(4,5‐dimethyldiazol‐2‐yl)‐2,5‐diphenyltetrazoliumbromide] solution (5 mg/mL) was added and the cells were stored in the dark at 37 °C until the appearance of a purple precipitate (formazan) [9 (link)]. Then, DMSO was added and the cells were left for 15 min; afterwards, the absorbance was measured using an Infinite F200 PRO microplate reader (Tecan Trading AG, Männedorf, Switzerland) at 550 nM. Images were captured using an EVOS XL Core Cell Imaging System (Life technologies, Thermo Fisher Scientific Korea Co., Ltd.).

Gyawali A., Latif S., Choi S.H., Hyeon S.J., Ryu H, & Kang Y.S. (2022). Monocarboxylate transporter functions and neuroprotective effects of valproic acid in experimental models of amyotrophic lateral sclerosis. Journal of Biomedical Science, 29, 2.

+ Open protocol

+ Expand

Neuroprotective Effects of Carnitine Compounds in ALS Cell Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

NSC-34 cell lines were exposed to glutamate with or without L-carnitine (LC) and acetyl L-carnitine (ALC) at a 1 mM concentration for 24 h. To assess the cell survival rate in ALS model cell lines, an MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazoliumbromide] assay was performed, following which [³H]L-carnitine uptake, and slc22a4 mRNA expression level were examined [22 (link)]. Cell lines were exposed to LC and ALC for 15 min, following which glutamate was added and incubated for 24 h. Next, MTT (5 mg/mL) solution was added to each well and stored at 37 °C in the dark for 3 h. After the solution turned purple due to precipitation of formazan, DMSO was added and absorbance was evaluated using an Infinite F200 PRO microplate reader (Tecan Trading AG, Männedorf, Switzerland) at a wavelength of 550 nM, as previously described [22 (link)]. Here, glutamate was used to induce glutamate-induced neurotoxicity, and LC and ALC were added to examine their neuroprotective effects on both the NSC-34 cell lines [17 (link)].

Gyawali A., Hyeon S.J., Ryu H, & Kang Y.S. (2021). The Alteration of L-Carnitine Transport and Pretreatment Effect under Glutamate Cytotoxicity on Motor Neuron-Like NSC-34 Lines. Pharmaceutics, 13(4), 551.

+ Open protocol

+ Expand

Evaluating Cytokine and Oxidative Stress Survival

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the survival rate of the cells after exposure to inflammatory cytokines and oxidative stressor, the MTT [3-(4, 5-dimethyldiazol-2-yl)-2, 5-diphenyltetrazoliumbromide] assay was conducted. ALS model cell lines were treated with glutamate (2 mM) and LPS (20 ng/mL) and H₂O₂ (300 µM), either alone or in combination with pretreatment of paeonol (100 µM) and incubated for 24 h. After the designated time, MTT (5 mg/mL) solution was added to each well, and the well plate was wrapped in the foil in the dark and incubated at 37 °C for 3 h. The formazan precipitation of MTT solution turned into the purple solution. The well plate was washed with phosphate-buffered saline (PBS) and dimethylsulfoxide was added. The absorbance was measured using an Infinite F200 PRO microplate reader (Tecan Trading AG, Männedorf, Switzerland) at a wavelength of 550 nM [23 (link)]. For the analysis of data, we compared the absorbance value in MT with the absorbance value in WT control treated with glutamate, H₂O₂, and LPS in the presence or absence of paeonol pretreatment.

Latif S., Choi S.H., Gyawali A., Hyeon S.J., Kang Y.S, & Ryu H. (2022). Antioxidant and Neuroprotective Effects of Paeonol against Oxidative Stress and Altered Carrier-Mediated Transport System on NSC-34 Cell Lines. Antioxidants, 11(7), 1392.

+ Open protocol

+ Expand

Quantification of THP-1 Cell Adhesion

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analysis of the THP-1 cell adhesion to a HUVEC monolayer under flow conditions was performed using an ibidi pump system (ibidi, Gräfelfing, Germany). Briefly, HUVECs (1.6 × 10⁶ cells/mL) were seeded into collagen G pre-coated μ-slide I^0.8 Luer channel slides (ibidi, Germany), let to attach for 2 h and subjected to 1 h of 1 dyn/cm², 1 h of 3 dyn/cm² and constant 5 dyn/cm² shear pressure overnight under cell culture conditions. After overnight cultivation and subsequent treatment as indicated in the respective figure legend, the monolayers were tested for the adhesion of THP-1 cells (8 x 10⁵ cells/mL), which were stained with 5 µM CellTracker Green (CTG; Life Technologies, Carlsbad, CA, USA) in serum-free RPMI for 30 min according to the manufacturer’s instructions. Adhesion was performed for 10 min at 0.5 dyn/cm² under cell culture conditions. Non-adherent THP-1 cells were removed by washing once with PBS containing Ca²⁺ and Mg²⁺ and the cells were lysed using RIPA and frozen at −80 °C. Quantification of the THP-1 cell adhesion was performed by fluorescence measurement (ex.: 485 nm, em.: 535 nm) of the obtained lysates by using an Infinite F200 pro microplate reader (Tecan Trading, Männedorf, Switzerland).

Primke T.F., Ingelfinger R., Elewa M.A., Macinkovic I., Weigert A., Fabritius M.P., Reichel C.A., Ullrich A., Kazmaier U., Burgers L.D, & Fürst R. (2023). The Microtubule-Targeting Agent Pretubulysin Impairs the Inflammatory Response in Endothelial Cells by a JNK-Dependent Deregulation of the Histone Acetyltransferase Brd4. Cells, 12(16), 2112.

+ Open protocol

+ Expand

Viability Assay of HUVECs

Check if the same lab product or an alternative is used in the 5 most similar protocols

HUVECs were seeded into a 96-well plate and were grown to confluence and treated as indicated. The metabolic activity was tested with the CellTiterBlue Cell Viability Assay (Promega, Germany) following the manufacturer’s instructions. The metabolism-dependent reduction of resazurin into resorufin was analyzed by fluorescence measurement (ex.: 579 nm, em.: 584 nm) using an Infinite F200 pro microplate reader (Tecan Trading, Switzerland).

+ Open protocol

+ Expand

Oxidative Stress Quantification Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells (1×10⁴) were seeded onto 96-well-plate (Corning, black plate, clear bottom) in quadruplicate per cell line in normoxic and in hypoxic conditions. Cells were treated with 400μM H₂O₂ 1,5h before the measurement as a positive control. Treated and non-treated cells were washed with PBS and incubated with 5μM 2′,7′dichlorofluorescein diacetate (DCF-DA-Sigma-Aldrich) for 30min at 37°C in the dark. DCF was then detected by an Infinite F200 PRO microplate reader (TECAN Trading AG, Switzerland) using excitation and emission wavelengths of 485 and 525 nm, respectively. Four independent experiments were performed and protein normalization was made after each experiment.

de Padua M.C., Delodi G., Vučetić M., Durivault J., Vial V., Bayer P., Noleto G.R., Mazure N.M., Ždralević M, & Pouysségur J. (2017). Disrupting glucose-6-phosphate isomerase fully suppresses the “Warburg effect” and activates OXPHOS with minimal impact on tumor growth except in hypoxia. Oncotarget, 8(50), 87623-87637.

+ Open protocol

+ Expand

Measuring Nitric Oxide Levels in MT Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The levels of NO released by MT cells were measured using the Griess reagent system (Promega, Madison, WI, USA) (Sasabe et al., 2007 (link); Jung et al., 2013 (link)). To examine the effect of several compounds on NO levels, MT cells were treated with 1 mM glutamate and/or co-treated with 20 mM L-citrulline, and L-arginine for 24 h. Then 100 μL of culture supernatant was collected from each sample (n=3) and added to a 96-well micro-plate. After 24 h of pre-treatment, the samples were incubated with 100 μL of modified Griess reagent (1% sulfanilamide in 5% phosphoric acid) at room temperature for 7 min. Finally, 0.1 N-1-napthylethylenediamine dihydrochloride in water was added followed by incubation at room temperature for 7 min. The absorbance was measured at 550 nm using an Infinite F200 PRO microplate reader (Tecan Trading AG, Männedorf, Swiss). A calibration curve was prepared using 0.1 M sodium nitrite in water as a standard.

Gautam S., Latif S, & Kang Y.S. (2024). Effect of Various Pathological Conditions on Nitric Oxide Level and L-Citrulline Uptake in Motor Neuron-Like (NSC-34) Cell Lines. Biomolecules & Therapeutics, 32(1), 154-161.

+ Open protocol

+ Expand

Antimicrobial Activity Screening Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysis assays were performed in sterile nontreated 96-well microplates (Brand, Wertheim, Germany) at 37°C with shaking (400 rpm) for 2 h. Heat-inactivated cells of S. aureus strain COL and M. luteus, were prepared as above, and were resuspended in 50 mmol/L Tris pH 8.0 to an initial OD₆₀₀ of 0.3. Low-molecular weight salmon sperm DNA and purified proteins AMR_1–2 and R₃GL were added to the wells at the beginning of the assay at different concentrations. Three biological replicates were performed in triplicate in an Infinite F200 PRO microplate reader (Tecan Group Ltd., Männedorf, Switzerland).
The decrease in OD₆₀₀ observed for each protein/DNA concentrations was calculated as a percentage of the initial OD₆₀₀ value.

Grilo I.R., Ludovice A.M., Tomasz A., de Lencastre H, & Sobral R.G. (2014). The glucosaminidase domain of Atl – the major Staphylococcus aureus autolysin – has DNA-binding activity. MicrobiologyOpen, 3(2), 247-256.

+ Open protocol

+ Expand

Splicing Regulation Assay in HEK293T

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK 293T cells were seeded in 24-well plates at a density of 9 × 10⁴ cells/well. After 24 h, the cells were cotransfected with 200 ng of the pTN24 reporter plasmid (88 (link)), MIY-UHMK1^WT and MIY-UHMK1^K54R (200 ng) construct alone or in combination with pcDNA-SF1(4) (300 ng of each construct). DNA was kept constant at 800 ng DNA in each well and 2 μl of Lipofectamine 2000 (Thermo Fisher Scientific) was used as a transfection reagent. The cells were harvested 24 h later and assayed for luciferase and

β

-Galactosidase activity using Dual Light Luciferase and

β

-Galactosidase Reporter Gene Assay System (Thermo Fisher Scientific). The measurements were performed in duplicates in an Infinite F200 Pro microplate reader (Tecan Group Ltd). Splicing was evaluated as a ratio of luciferase/

β

-galactosidase. Parallel transfections were performed in the same conditions to assess gene expression of UHMK1^WT, UHMK1^K54R, and SF1 by qPCR.

Arfelli V.C., Chang Y.C., Bagnoli J.W., Kerbs P., Ciamponi F.E., Paz L.M., Pankivskyi S., de Matha Salone J., Maucuer A., Massirer K.B., Enard W., Kuster B., Greif P.A, & Archangelo L.F. (2023). UHMK1 is a novel splicing regulatory kinase. The Journal of Biological Chemistry, 299(4), 103041.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!