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2 protocols using ab37615

1

Comprehensive Antibody Panel for Alzheimer's Research

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Anti-M6PR (cation independent) antibody [2G11] (ab2733; Abcam).
Anti-APP (A8717; Sigma).
Anti-APP 6e10 to detect Aβ, sAPPα (SIG-39320; Covance).
Anti-GAPDH (Sigma).
Anti-tubulin (Sigma).
Anti-cyclophilin B (ACB0825; Atgen).
Anti-sAPPβ Swedish (6A-1; IBL).
Anti-VPS35 (SC-374372; Santa Cruz).
Anti-LAMP-1 (SC-18821; Santa Cruz).
Anti-PLD3 (HPA012800; Sigma).
Anti-transferrin receptor (13-6800; Invitrogen).
Anti-SORL1 (612633; BD Bioscience).
Anti-GFP (Seaman lab).
Anti-LBPA (MABT837; Sigma).
Anti-EEA1 (610456; BD Biosciences).
Anti-GM130 (610822; BD Biosciences).
Anti-TGN46 (Seaman lab).
Anti-MICALL1 (H00085377-B01P, Novus).
Anti-SNAP29 (gift from Andrew Peden, University of Sheffield, UK).
Anti-PACSIN2 (ab37615, Abcam).
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2

Antibody Profiling of Centrosomal Proteins

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Commercial antibodies used were as follows: anti-Acetylated tubulin (Actub, clone 6-11B-1, 1/10000, T6793, Sigma), anti-Gamma-tubulin (GTU-88, 1/1000, T6557, Sigma), anti-b-actin (clone AC-15, 1/30000, A5441, Sigma), anti-PACSIN1 (1/100, 196 003, Synaptic Systems), anti-PACSIN2 (1/250, ab37615, Abcam), anti-PACSIN2 (1/500, 10518-2-AP, Proteintech), anti-PACSIN3 (1/100, ab37612, Abcam), anti-Pericentrin (PCTN, 1/5000, NB100-61071, Novus Biologicals), anti-EHD1 (EPR4954, 1/500, ab109311, Novus Biologicals), anti-RPGRIP1L (1/200, 55160-1-AP, Proteintech), anti-TMEM67 (1/200, 12780-1-AP, Proteintech), anti-CEP164 (1/500, sc-240226, Santa Cruz), anti-CP110 (1/1000, 12780-1-AP, Proteintech), anti-CEP97 (1/1000, A301-945A, Bethyl), anti-Arl13b (1/300, clone N295B/66, NeuroMab/UC Davis), anti-GFP Alexa 488 (1/1000, A21311, Molecular Probes Life Technologies), Phalloidin conjugated with Alexa 488 (1/50, A12379, Molecular Probes Life Technologies), Hoechst (1/3000, H3570, Molecular Probes Life Technologies) and all secondary antibodies were from Life Technologies. The rabbit anti-RAB8A antibody was a gift from Johan Peränen (University Helsinki, Finland). SNAP-Cell647-SiR reagent was purchased from New England Biolabs. Nocodazole (Calbiochem) was from Sigma. Doxycyclin hydrochloride was obtained from Sigma and used according to manufacturer’s instruction.
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