MiScript miRNA mimics and inhibitors were purchased from Qiagen. AllStars negative mimics (Qiagen) were used as controls. Transient transfections were performed using Lipofectamine 2000 (Life Technologies) according to manufacturer’s instructions. Day 7 differentiated mD-Mφ were transfected at a final concentration of 50 nM. Red siGLO oligos (Thermo Fisher Scientific, Waltham, MA, USA) were used to confirm successful transfection, with transfection efficiency being greater than 90% as previously reported [9 (link)]. Cell viability was assessed using the CellTiter 96 AQueous Cell Proliferation Assay Kit (Promega, Madison, WI, USA). Viability of all cell culture samples was analyzed 2 hours prior to harvesting of cells/supernatant for subsequent experimental analysis.
Miscript mirna mimics and inhibitors
Manufactured by Qiagen
The MiScript miRNA mimics and inhibitors are laboratory tools designed to modulate the expression of microRNA (miRNA) molecules. MiScript mimics are synthetic RNA molecules that mimic the sequence and function of a specific endogenous miRNA, while MiScript inhibitors are designed to block the activity of a target miRNA. These products are intended for research use only and their core function is to facilitate the study of miRNA-mediated gene regulation in various biological systems.
Automatically generated - may contain errors
2 protocols using miscript mirna mimics and inhibitors
1
miRNA Mimic Transfection in Differentiated mD-Mφ
2
miRNA Mimic Transfection in Differentiated mD-Mφ
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MiScript miRNA mimics and inhibitors were purchased from Qiagen. AllStars negative mimics (Qiagen) were used as controls. Transient transfections were performed using Lipofectamine 2000 (Life Technologies) according to manufacturer’s instructions. Day 7 differentiated mD-Mφ were transfected at a final concentration of 50 nM. Red siGLO oligos (Thermo Fisher Scientific, Waltham, MA, USA) were used to confirm successful transfection, with transfection efficiency being greater than 90% as previously reported [9 (link)]. Cell viability was assessed using the CellTiter 96 AQueous Cell Proliferation Assay Kit (Promega, Madison, WI, USA). Viability of all cell culture samples was analyzed 2 hours prior to harvesting of cells/supernatant for subsequent experimental analysis.
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