AFM measurements were carried out on a Nanoscope IV multimode AFM (Veeco Instruments Inc.). Images were generated in the PeakForce QNM (quantitative nanomechanical property mapping) mode with a silicon oxide tip (Olympus microcantilever OTR8 PS-W) having a spring constant of 0.15 N/m and a radius of curvature of <20 nm. AFM imaging was performed at room temperature (~25°C) on freshly cleaved mica surfaces. A liquid flow cell (glass probe holder, MTFML, Bruker Corporation) was used to scan the surfaces in a liquid environment and to exchange solution in situ. All images were recorded at a resolution of 512 × 512 pixels and with a scan rate of 1 Hz. The z-set point and differential gains were manually optimized during each scan. Images were analyzed and processed in the Gwyddion 2.22 software.
Otr8 ps w
Manufactured by Olympus
Sourced in United States
The OTR8 PS-W is a laboratory equipment product from Olympus. It is a precision-engineered device designed for scientific applications. The core function of the OTR8 PS-W is to perform specific tasks within a controlled laboratory environment. No further details can be provided while maintaining an unbiased and factual approach.
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Lab products found in correlation
3 protocols using otr8 ps w
1
Quantitative Nanomechanical Mapping of Surfaces
2
Silica Particle Attachment to Cantilever
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A light microscope (BX51, Olympus) and a micromanipulator (model
MMO-202D, Narishige) were used to attach a silica particle (nominal
diameter = 6.84 μm, Bangs Laboratory, Fishers, USA) to a gold-plated
Si3N4 cantilever (V-shaped, nominal spring constant k = 0.15 N/m, OTR8–PS-W, Olympus) with an epoxy resin
(Araldite Rapid). The silica probe was next modified with hydrophilpos using the method reported elsewhere.5 (link)
MMO-202D, Narishige) were used to attach a silica particle (nominal
diameter = 6.84 μm, Bangs Laboratory, Fishers, USA) to a gold-plated
Si3N4 cantilever (V-shaped, nominal spring constant k = 0.15 N/m, OTR8–PS-W, Olympus) with an epoxy resin
(Araldite Rapid). The silica probe was next modified with hydrophilpos using the method reported elsewhere.5 (link)
3
Atomic Force Microscopy of Supported Lipid Bilayers
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Atomic force microscopy measurements were carried out on a Nanoscope IV multimode AFM (Veeco Instruments Inc.). Images were generated in the PeakForce QNM (quantitative nanomechanical property mapping) mode with a silicon oxide tip (Olympus microcantilever OTR8 PS-W) with a spring constant of 0.15 N/m and a radius of curvature <20 nm. A freshly cleaved mica surface was imaged in ultrapure water to ensure a clean and smooth surface (RMS < 500 pm) prior to SLB formation. AFM imaging of the bilayer formation process was carried out under continuous flow of the vesicle solution at room temperature as described previously [20] using a slow gravity-fed flow of approximately 50 μL/min. After bilayer formation in ultrapure water, the membranes were rinsed with buffer. All images were recorded at a resolution of 512 × 512 pixels and with a scan rate of 1 Hz. The z-set point and differential gains were optimized during each scan. Images were analyzed and processed in the Gwyddion 2.22 software.
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