Poly l lysine coated glass slides

Cochlear sections were obtained from CBA/CaJ mice. Temporal bones were removed from P5-7 mice and placed in either 10% formalin or 100% methanol for 45 min followed by three rinses with 0.01M phosphate-buffered saline (PBS, pH7.4) for 15 min or overnight at room temperature (RT). Temporal bones were dehydrated in 50%, 75%, 80% and 95% ethanol for 1 hr each, followed by 95% ethanol for an additional hour, 100% ethanol for 30 min, and n-Butanol overnight at RT. The next day, n-Butanol was replaced with fresh n-Butanol, which was applied for an additional 2 hours. Finally, temporal bones were embedded in paraffin, sectioned at 4–6 µm thickness, placed on poly-l-lysine coated glass slides (VWR, 48311-703), dried overnight and stored at RT until ready for use.

IR-780 iodide, glycerol, NaIO4 and Bovine serum albumin (BSA) were obtained from the Sigma-Aldrich. The 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4 Chlorobenzenesulfonate Salt (DiD) and 4,6-diamidino-2 phenylindole (DAPI) stain were ordered from Invitrogen. Aldehyde Quantification Assay Kit (Fluorometric) was from Abcam. Poly -L -lysine coated glass slides were obtained from VWR International Inc..Walgreens sunscreen lotion (SPF 30,, Avobenzone 3.0%, Homosalate 10.0%, Octisalate 5.0%, Octocrylene 10%) was purchased from Walgreens. Sunscreen oil (SPF 30, Avobenzone 3.0%, Homosalate 10.0%, Octisalate 5.0%, Octocrylene 7%) was from L'Oréal Paris.