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2 protocols using regenerated cellulose membrane filters

1

Quantitative Analysis of Cationic Surfactants

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BEC (99%), DDAB (98%), BAC12 (puriss, ≥99 %), BAC14 (puriss, ≥99 %), BAC16 (99%), hexadecyltrimethylammonium bromide (HDTAB, 99.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HDTAB was used as an internal standard (ISTD) for BEC and DDAB. Deuterated ISTDs (BAC12-d5, BAC14-d5, and BAC16-d5, 99%) were purchased from CDN Isotopes Inc. (Pointe Claire, Quebec). LCMS-grade acetonitrile, 2-propanol, formic acid, and ammonium formate were purchased from Thermo Scientific (Waltham, MA, USA). Water (18 MΩ) was cleaned by a Millipore Milli-Q system (Billerica, MA, USA). Polypropylene centrifuge tubes (15 ml) were purchased from Fisher Scientific. All samples and standards were passed through regenerated cellulose membrane filters (0.2-μm pore size, 15-mm i.d., Phenomenex, Torrance, CA, USA).
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2

Cell Culture Protocol with Optimization

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RPMI-1640 growth medium supplemented with L-glutamine and glucose oxidase (from Aspergillus niger, 18,000 units/g) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine calf serum (FBS), Nunc tissue culture plastics, low melting-point agarose and penicillin/streptomycin antibiotics were obtained from Gibco GRL (Grand Island, NY, USA). Microscope superfrosted glass-slides were supplied by Menzel-Glaset (Menzel, Germany). Calcein-AM was from Molecular Probes (Eugene, OR, USA). Formic acid (LC-MS grade, 98%) was obtained from Fluka. Regenerated cellulose membrane filters with 0.2 µm pore size and 4 mm diameter were purchased from Phenomenex. A Kinetex C18 column 100 mm × 2.1 mm, 2.6 µm column was purchased from Phenomenex, USA. The specific iron chelator SIH (salicylaldehyde isonicotinoyl hydrazone) was a kind donation from Professor Prem Ponka (McGill University, Montreal, QC, Canada). All other chemicals used were of analytical grade.
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