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Black opaque 96 well plate

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The black opaque 96-well plate is a laboratory equipment designed for various applications. It features a high-density well arrangement, allowing for efficient use of sample volumes. The plate's black opaque construction helps to minimize well-to-well optical interference and background fluorescence, making it suitable for diverse assays and experiments.

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Lab products found in correlation

Spectramax m2e, by Molecular Devices (2 mentions) Spectramax m2e microplate reader, by Molecular Devices (1 mentions) One glo substrate, by Promega (1 mentions) Fluorescence spectrophotometer, by Agilent Technologies (1 mentions) Dibac4 3, by Merck Group (1 mentions) White opaque 96 well plate, by Corning (1 mentions)

Close spelling variants of this product

96-well plates (3030 citations) 96 wells (10 citations) Black opaque (4 citations)

6 protocols using black opaque 96 well plate

1

OMA1 Activity Assay for Mitochondria

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OMA1 activity assay was performed by the technique developed recently [25 (link)]. The assay utilizes an OPA1 fluorogenic reporter substrate that contains a fluorogenic MCA moiety on the N-terminus and a DNP quencher moiety on the C-terminus (LifeTein LLC). Briefly, 5 μg of each mitochondrial sample was resolved in the OMA1 activity assay buffer, containing (in mM): 50 Tris-HCl and 40 KCl, pH 7.5. Reaction medium contained 200 μM N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine (the zinc chelator) and 5 μM OPA1 fluorogenic reporter substrate. The assay was run with a final volume of 100 μL using black opaque 96 well plates (Costar). Relative fluorescence was recorded at 37°C in 5-min intervals for 30-min, using a fluorescent plate reader (SpectraMax M2e, Molecular Devices equipped with SoftMax Pro v5 software) with excitation/emission of 320/405 nm. OMA1 activation was proportional to the fluorescence released as a result of cleavage of the OPA1 fluorogenic reporter substrate by OMA1. Results are presented in relative fluorescence units.
Rodríguez-Graciani K.M., Chapa-Dubocq X.R., MacMillan-Crow L.A, & Javadov S. (2020). Association Between L-OPA1 Cleavage and Cardiac Dysfunction During Ischemia-Reperfusion Injury in Rats. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 54(6), 1101-1114.
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2

OMA1 Activity Assay for Mitochondria

Cited 1 time
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OMA1 activity assay was performed by the technique developed recently [25 (link)]. The assay utilizes an OPA1 fluorogenic reporter substrate that contains a fluorogenic MCA moiety on the N-terminus and a DNP quencher moiety on the C-terminus (LifeTein LLC). Briefly, 5 μg of each mitochondrial sample was resolved in the OMA1 activity assay buffer, containing (in mM): 50 Tris-HCl and 40 KCl, pH 7.5. Reaction medium contained 200 μM N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine (the zinc chelator) and 5 μM OPA1 fluorogenic reporter substrate. The assay was run with a final volume of 100 μL using black opaque 96 well plates (Costar). Relative fluorescence was recorded at 37°C in 5-min intervals for 30-min, using a fluorescent plate reader (SpectraMax M2e, Molecular Devices equipped with SoftMax Pro v5 software) with excitation/emission of 320/405 nm. OMA1 activation was proportional to the fluorescence released as a result of cleavage of the OPA1 fluorogenic reporter substrate by OMA1. Results are presented in relative fluorescence units.
Rodríguez-Graciani K.M., Chapa-Dubocq X.R., MacMillan-Crow L.A, & Javadov S. (2020). Association Between L-OPA1 Cleavage and Cardiac Dysfunction During Ischemia-Reperfusion Injury in Rats. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 54(6), 1101-1114.
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3

Quantifying GFP Fluorescence in Bacteria

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To quantify the green fluorescence intensity of the GFP, 1 ml of GFP expressing bacterial cells were harvested, washed and re-suspended in 500 μl 1X PBS (phosphate buffered saline). Fluorescence intensities of the re-suspended cells were measured in a black opaque 96 well plate (Corning®, USA) at excitation of 490 nm and emission of 510 nm using the SpectraMax M2e microplate reader (Molecular Device, USA). Fluorescence values were normalized with optical density of the bacterial cells.
Srivastava S.K., Iyer V.R., Ghosh T., Lambadi P.R., Pathania R, & Navani N.K. (2016). Isolation of a non-genomic origin fluoroquinolone responsive regulatory element using a combinatorial bioengineering approach. Nucleic Acids Research, 44(5), 2451-2461.
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4

Cytotoxicity Evaluation of Compounds in A549-hACE2 Cells

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The cytotoxicity of compounds was determined in A549-hACE2 cells in the following manner. A549-hACE2 cells (12,000 cells per well in medium containing 2% FBS) were plated into a black opaque 96-well plate (Corning). The next day, 3-fold serial dilutions of compounds were prepared in DMSO in a manner to normalize the DMSO concentration among all cell culture wells. The compounds were further diluted 500-fold in the culture medium containing 2% FBS. Spent medium from overnight incubation was aspirated, 100 μl of diluted compound solutions were added to each well, and cultures were returned to 37°C with 5% CO2. After 48 h, 100 μl OneGlo substrate (Promega) was added to each well. Luciferase signals were measured using an Envision microplate reader. The relative luciferase signals were calculated by normalizing the luciferase signals of the compound-treated groups to that of the DMSO-treated groups (set as 100%). The relative luciferase signal (y axis) versus the compound concentration (x axis) was plotted in software GraphPad Prism 8 (version 8). The compound concentration for reducing 50% of luciferase signal as a measure of cell viability (CC50) values were calculated using a nonlinear 4-parameter regression model. Three independent experiments were performed with technical duplicates.
Li R., Liclican A., Xu Y., Pitts J., Niu C., Zhang J., Kim C., Zhao X., Soohoo D., Babusis D., Yue Q., Ma B., Murray B.P., Subramanian R., Xie X., Zou J., Bilello J.P., Li L., Schultz B.E., Sakowicz R., Smith B.J., Shi P.Y., Murakami E, & Feng J.Y. (2021). Key Metabolic Enzymes Involved in Remdesivir Activation in Human Lung Cells. Antimicrobial Agents and Chemotherapy, 65(9), e00602-21.
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5

Measuring Bacterial Membrane Potentials

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Cell membrane potentials were determined using the fluorescence dye bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4 (3); Sigma, St. Louis, USA) according to methods described elsewhere [22 (link)] with minor modifications. Cells of MDR S. aureus SA-596 and MDR P. aeruginosa PA-69 were prepared as described in Section 2.6.1. Each pellet was resuspended in PBS (108 CFU/mL) with one of the various concentrations of verbascoside (0, MIC, or 2 × MIC). Then, the samples were incubated for 30 min at 30 °C. A bacterial suspension (200 μL) and the fluorescent probe DiBAC4 (3) (1 mM final concentration) were added to each well of a black, opaque 96-well plate (Corning, NY, USA). The mixtures were then incubated at 37 °C for 30 min in the dark. Cell suspensions were analysed using a fluorescence spectrophotometer (BioTek, Winooski, VT, USA) with excitation and emission wavelengths of 488 nm and 518 nm, respectively. Changes in the fluorescence intensity of DiBAC4 (3) were recorded to monitor the membrane potential. All the values were corrected for cell number and background fluorescence.
Shi C., Ma Y., Tian L., Li J., Qiao G., Liu C., Cao W, & Liang C. (2022). Verbascoside: An Efficient and Safe Natural Antibacterial Adjuvant for Preventing Bacterial Contamination of Fresh Meat. Molecules, 27(15), 4943.
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6

HaloTag Transcription Activation Assay

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0.5–0.6 ×105 HEK 293T cells were seeded in each well of a 48-well plate. 24 h later, the cells were transfected with 120 ng of the designated HaloTag fusion gene plasmid and 120 ng of 40:1 FHRE::firefly luciferase: pCMV::Renilla luciferase, using TransIT–2020 transfection agent. 24 h post-transfection, cells were treated with DMSO or 25 μM Ht-PreHNE for 2.5 h, rinsed three times and irradiated with 365 nm UV light for 3 min. The cells were incubated for 18 h after which the cells were trypsinized, washed two times and lysed in 65 μL of 1X passive lysis buffer (see Luciferase assay protocol). 20 μL of the lysate was transferred to a white opaque 96-well plate (Corning). Firefly luciferase and Renilla luciferase activities (the latter was used for normalization) were measured as described (see Luciferase assay protocol). FHRE assay data were consistent with those shown in Supplementary Figure S6. 25 μL of the remaining lysate was transferred to a black opaque 96-well plate (Corning) for measuring caspase activity. 100 μL caspase substrate containing 50mM HEPES (7.4), 100mM NaCl, 0.1% CHAPS, 10mM DTT, 1mM EDTA, 10% glycerol and 15 μM Ac-DEVD-AMC was added to each well and the release of AMC was measured continuously by fluorescence for 2 h at 37 °C using a plate reader with excitation at 380 nm and emission at 440 nm.
Long M.J., Parvez S., Zhao Y., Surya S.L., Wang Y., Zhang S, & Aye Y. (2017). Akt3 is a privileged first responder in isozyme-specific electrophile response. Nature chemical biology, 13(3), 333-338.
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