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Be0027

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The BE0027 is a laboratory centrifuge. It is designed to separate and isolate samples, cells, or other materials based on their density and molecular weight. The centrifuge operates at high speeds to generate a strong centrifugal force, which enables the separation of different components within a sample.

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Lab products found in correlation

Be0088, by BioXCell (4 mentions) Be0071, by BioXCell (2 mentions) Sc 17320, by Santa Cruz Biotechnology (2 mentions) Mca1957, by Bio-Rad (2 mentions) Ab19569, by Abcam (2 mentions) Ab6326, by Abcam (2 mentions) Ve cadherin, by Santa Cruz Biotechnology (2 mentions) Mab360mi, by Vector Laboratories (2 mentions) Ab2218, by Bio-Rad (2 mentions) Mab002 clone 11711, by BioXCell (2 mentions) Sc 8066, by Santa Cruz Biotechnology (2 mentions) Alzet 1002, by Durect (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

8 protocols using be0027

1

Investigating VCAM1 and VLA-4 Modulation

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Aged (16-month-old) C57BL6/J mice received i.p. injections of anti-VCAM1 mAb anti-VCAM1 antibody (BE0027, BioXcell) or IgG isotype control (9 mg/kg) every 3 days for a total of 7 injections. Mice also received BrdU daily (100 mg/kg i.p.) for the last 6 days prior to perfusion 24 hours after the last BrdU injection and 48 hours after the last antibody injection.
Similarly, aged (16-month-old) C57BL6/J mice received i.p. injections of anti-VLA-4 mAb (BE0071, BioXCell) or IgG isotype control (9 mg/kg) every 3 days for a total of 7 injections. Mice also received BrdU daily (100 mg/kg i.p.) for the last 6 days prior to perfusion 24 hours after the last BrdU injection and 48 hours after the last antibody injection.
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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2

Multimodal Immunofluorescence Staining Protocol

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Rat monoclonal anti-BrdU (1:500, Abcam, ab6326, clone BU1/75[ICR1])
Rat monoclonal anti-VCAM1 (1:125, Abcam, ab19569, clone M/K-2)
Goat monoclonal anti-Sox2 (1:100, Santa Cruz, sc-17320, clone Y-17)
Goat polyclonal anti-doublecortin (DCX) (1:100, Santa Cruz, sc-8066, clone C-18)
Goat polyclonal VE-Cadherin (Santa Cruz Biotechnology, sc-6458, clone C-19)
Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit (Thermo/Life Technologies, C-10637)
Mouse monoclonal anti-GFAP (1:1000, Chemicon/Fisher, MAB360MI, clone GA5)
DyLight 488 Lectin (1:200, Vector, DL-1174)
Rabbit monoclonal anti-Aquaporin 4 (1:500, Millipore, AB2218, clone)
Rat monoclonal anti-CD68 (1:600, Serotec, MCA1957, clone FA-11)
Rabbit polyclonal anti-Iba1 (1:250, ProteinTech, 10904–1-AP)
Mouse anti-human-VCAM1 antibody (Novus Biologicals, BBA5, clone BBIG-V1)
Mouse monoclonal anti-human IgG antibody (R&D Systems, MAB002 clone 11711)
Rat monoclonal anti-VCAM-1 (BioxCell, BE0027, clone M/K-2.7)
Rat IgG1 Isotype antibody (BioxCell, BE0088, clone HRPN)
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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3

Anti-VCAM1 mAb Evaluation in Aging Mice

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Aged (17-month-old) or adult (5-month-old) C57BL6/J male mice were injected with anti-VCAM1 mAb (BioXCell cat # BE0027) or IgG isotype control (BioXCell cat # BE0088), 9 mg/kg i.p. every 3 days for a total of 8 injections over a 3 week period. Behavioral testing began at the start of week 4, and antibody injections continued every 3 days throughout the testing period.
13-month-old NSG mice (n=11 mice per group) were injected with anti-VCAM1 mAb or IgG1 isotype control (9 mg/kg i.p) every 3 days for nine injections over 4 weeks. Behavior experiments were performed between 7th and 9th injection of antibodies during the last week.
23-month-old C57BL6 males (“WT”) were treated with anti-VCAM1 (n=13), IgG (n=12), PBS (n=7). They each received 10 i.p. injections (9 mg/kg) of antibody every 3 days for 4 weeks. Behavior experiments were performed between 8th and 10th injection of antibodies.
Behavior testing was performed in a controlled environment as previously described78 ,79 . Tests were performed in groups of 4 mice at a time with even lighting conditions (30 ± 5 lumens). All trials were recorded using a ceiling mounted camera and Topscan Version 2.00 Software. All equipment was thoroughly cleaned using 70% Ethanol between trials.
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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4

Anti-VCAM-1 Antibody Attenuates Hypertensive Cardiac Remodeling

Cited 1 time
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All experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of the First Affiliated Hospital of Dalian Medical University (AEE20027). The wild-type (WT) male C57BL/6J mice were fed under specific pathogen-free conditions for 8–10 weeks. Mice were anesthesia with 1.5% isoflurane, then the osmotic pumps (Alzet, 1,002; DURECT, Cupertino, CA, United States) were subcutaneously implanted to deliver angiotensin (Ang) II (1,000 ng kg−1 min−1) for 14 days to construct a hypertensive cardiac remodeling model. Then anti-VCAM-1 neutralizing antibody (0.1 or 0.2 mg, BE0027, Bioxcell) or control IgG was intraperitoneally (i.p.) injected every 2 days as previously described (Yin et al., 2022 (link)).
Qiu Z.Y., Yu W.J., Bai J, & Lin Q.Y. (2022). Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration. Frontiers in Pharmacology, 13, 1058268.
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5

Multimodal Immunofluorescence Staining Protocol

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Rat monoclonal anti-BrdU (1:500, Abcam, ab6326, clone BU1/75[ICR1])
Rat monoclonal anti-VCAM1 (1:125, Abcam, ab19569, clone M/K-2)
Goat monoclonal anti-Sox2 (1:100, Santa Cruz, sc-17320, clone Y-17)
Goat polyclonal anti-doublecortin (DCX) (1:100, Santa Cruz, sc-8066, clone C-18)
Goat polyclonal VE-Cadherin (Santa Cruz Biotechnology, sc-6458, clone C-19)
Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit (Thermo/Life Technologies, C-10637)
Mouse monoclonal anti-GFAP (1:1000, Chemicon/Fisher, MAB360MI, clone GA5)
DyLight 488 Lectin (1:200, Vector, DL-1174)
Rabbit monoclonal anti-Aquaporin 4 (1:500, Millipore, AB2218, clone)
Rat monoclonal anti-CD68 (1:600, Serotec, MCA1957, clone FA-11)
Rabbit polyclonal anti-Iba1 (1:250, ProteinTech, 10904–1-AP)
Mouse anti-human-VCAM1 antibody (Novus Biologicals, BBA5, clone BBIG-V1)
Mouse monoclonal anti-human IgG antibody (R&D Systems, MAB002 clone 11711)
Rat monoclonal anti-VCAM-1 (BioxCell, BE0027, clone M/K-2.7)
Rat IgG1 Isotype antibody (BioxCell, BE0088, clone HRPN)
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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6

Angiotensin II-induced Hypertensive Model

Cited 5 times
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Wild-type (WT) C57BL/6J mice (male, 8–10 weeks) were acquired from The Jackson Laboratory. A hypertensive model was constructed by infusion of Ang II at a dosage of 490 ng kg−1·min−1 for 14 days as previously described (Wang et al., 2016 (link); Ji et al., 2017 (link); An et al., 2018 (link)). WT mice were intraperitoneally injected with 0.1 or 0.2 mg of anti-VCAM-1 neutralizing antibody (BE0027, Bioxcell) or IgG control every 2 days after Ang II infusion as previously reported (Thomas et al., 2011 (link); Chow et al., 2013 (link)). The animals were housed under specific pathogen-free (SPF) conditions and fed a regular diet. The experimental processes were authorized by the Institutional Animal Care and Use Committee (IACUC) at the First Affiliated Hospital of Dalian Medical University (AEE20027).
Yin L., Bai J., Yu W.J., Liu Y., Li H.H, & Lin Q.Y. (2022). Blocking VCAM-1 Prevents Angiotensin II-Induced Hypertension and Vascular Remodeling in Mice. Frontiers in Pharmacology, 13, 825459.
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7

Investigating VCAM1 and VLA-4 Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aged (16-month-old) C57BL6/J mice received i.p. injections of anti-VCAM1 mAb anti-VCAM1 antibody (BE0027, BioXcell) or IgG isotype control (9 mg/kg) every 3 days for a total of 7 injections. Mice also received BrdU daily (100 mg/kg i.p.) for the last 6 days prior to perfusion 24 hours after the last BrdU injection and 48 hours after the last antibody injection.
Similarly, aged (16-month-old) C57BL6/J mice received i.p. injections of anti-VLA-4 mAb (BE0071, BioXCell) or IgG isotype control (9 mg/kg) every 3 days for a total of 7 injections. Mice also received BrdU daily (100 mg/kg i.p.) for the last 6 days prior to perfusion 24 hours after the last BrdU injection and 48 hours after the last antibody injection.
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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8

Anti-VCAM1 mAb Evaluation in Aging Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aged (17-month-old) or adult (5-month-old) C57BL6/J male mice were injected with anti-VCAM1 mAb (BioXCell cat # BE0027) or IgG isotype control (BioXCell cat # BE0088), 9 mg/kg i.p. every 3 days for a total of 8 injections over a 3 week period. Behavioral testing began at the start of week 4, and antibody injections continued every 3 days throughout the testing period.
13-month-old NSG mice (n=11 mice per group) were injected with anti-VCAM1 mAb or IgG1 isotype control (9 mg/kg i.p) every 3 days for nine injections over 4 weeks. Behavior experiments were performed between 7th and 9th injection of antibodies during the last week.
23-month-old C57BL6 males (“WT”) were treated with anti-VCAM1 (n=13), IgG (n=12), PBS (n=7). They each received 10 i.p. injections (9 mg/kg) of antibody every 3 days for 4 weeks. Behavior experiments were performed between 8th and 10th injection of antibodies.
Behavior testing was performed in a controlled environment as previously described78 ,79 . Tests were performed in groups of 4 mice at a time with even lighting conditions (30 ± 5 lumens). All trials were recorded using a ceiling mounted camera and Topscan Version 2.00 Software. All equipment was thoroughly cleaned using 70% Ethanol between trials.
Yousef H., Czupalla C.J., Lee D., Chen M.B., Burke A.N., Zera K.A., Zandstra J., Berber E., Lehallier B., Mathur V., Nair R.V., Bonanno L.N., Yang A.C., Peterson T., Hadeiba H., Merkel T., Körbelin J., Schwaninger M., Buckwalter M.S., Quake S.R., Butcher E.C, & Wyss-Coray T. (2019). Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nature medicine, 25(6), 988-1000.
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